Inhibition of caspase-8 cascade restrains the osteoclastogenic fate of bone marrow cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F24%3A00588031" target="_blank" >RIV/67985904:_____/24:00588031 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16170/24:43881230

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s00424-024-02977-2" target="_blank" >https://link.springer.com/article/10.1007/s00424-024-02977-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00424-024-02977-2" target="_blank" >10.1007/s00424-024-02977-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Inhibition of caspase-8 cascade restrains the osteoclastogenic fate of bone marrow cells

Popis výsledku v původním jazyce

Osteoclasts are multinucleated cells of hematopoietic origin, with a pivotal role in bone development and remodeling. Failure in osteoclast differentiation and activation leads to various bone disorders, thus, attention has focused on a search of molecules involved in osteoclast regulatory pathways. Caspase-8 appears to be an interesting candidate for further exploration, due to its potential function in bone development and homeostasis. Mouse bone marrow cells were differentiated into osteoclasts by RANKL stimulation. Increased activation of caspase-8 and its downstream executioner caspases (caspase-3 and caspase-6) was found during osteoclastogenesis. Subsequent inhibition of caspase-8, caspase-3, or caspase-6, respectively, during osteoclast differentiation showed distinct changes in the formation of TRAP-positive multinucleated cells and reduced expression of osteoclast markers including Acp5, Ctsk, Dcstamp, and Mmp9. Analysis of bone matrix resorption confirmed significantly reduced osteoclast function after caspase inhibition. The results clearly showed the role of caspases in the proper development of osteoclasts and contributed new knowledge about non-apoptotic function of caspases.

Název v anglickém jazyce

Inhibition of caspase-8 cascade restrains the osteoclastogenic fate of bone marrow cells

Popis výsledku anglicky

Osteoclasts are multinucleated cells of hematopoietic origin, with a pivotal role in bone development and remodeling. Failure in osteoclast differentiation and activation leads to various bone disorders, thus, attention has focused on a search of molecules involved in osteoclast regulatory pathways. Caspase-8 appears to be an interesting candidate for further exploration, due to its potential function in bone development and homeostasis. Mouse bone marrow cells were differentiated into osteoclasts by RANKL stimulation. Increased activation of caspase-8 and its downstream executioner caspases (caspase-3 and caspase-6) was found during osteoclastogenesis. Subsequent inhibition of caspase-8, caspase-3, or caspase-6, respectively, during osteoclast differentiation showed distinct changes in the formation of TRAP-positive multinucleated cells and reduced expression of osteoclast markers including Acp5, Ctsk, Dcstamp, and Mmp9. Analysis of bone matrix resorption confirmed significantly reduced osteoclast function after caspase inhibition. The results clearly showed the role of caspases in the proper development of osteoclasts and contributed new knowledge about non-apoptotic function of caspases.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/GA21-21409S" target="_blank" >GA21-21409S: Fyziologické vlastnosti a funkce kmenových buněk vztahujících se k dentici se zaměřením na kontext in vivo</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Pflugers Archiv - European Journal of Physiology

ISSN

0031-6768

e-ISSN

1432-2013

Svazek periodika

476

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

14

Strana od-do

1289-1302

Kód UT WoS článku

001238578900001

EID výsledku v databázi Scopus

2-s2.0-85195415210