Efficient derivation of functional astrocytes from human induced pluripotent stem cells (hiPSCs)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F24%3A00603154" target="_blank" >RIV/67985904:_____/24:00603154 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/24:00138840 RIV/00669806:_____/24:10488424 RIV/00216208:11140/24:10488424

Výsledek na webu

<a href="https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0313514" target="_blank" >https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0313514</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0313514" target="_blank" >10.1371/journal.pone.0313514</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Efficient derivation of functional astrocytes from human induced pluripotent stem cells (hiPSCs)

Popis výsledku v původním jazyce

Astrocytes are specialized glial cell types of the central nervous system (CNS) with remarkably high abundance, morphological and functional diversity. Astrocytes maintain neural metabolic support, synapse regulation, blood-brain barrier integrity and immunological homeostasis through intricate interactions with other cells, including neurons, microglia, pericytes and lymphocytes. Due to their extensive intercellular crosstalks, astrocytes are also implicated in the pathogenesis of CNS disorders, such as ALS (amyotrophic lateral sclerosis), Parkinson's disease and Alzheimer's disease. Despite the critical importance of astrocytes in neurodegeneration and neuroinflammation are recognized, the lack of suitable in vitro systems limits their availability for modeling human brain pathologies. Here, we report the time-efficient, reproducible generation of astrocytes from human induced pluripotent stem cells (hiPSCs). Our hiPSC-derived astrocytes expressed characteristic astrocyte markers, such as GFAP, S100b, ALDH1L1 and AQP4. Furthermore, hiPSC-derived astrocytes displayed spontaneous calcium transients and responded to inflammatory stimuli by the secretion of type A1 and type A2 astrocyte-related cytokines.

Název v anglickém jazyce

Efficient derivation of functional astrocytes from human induced pluripotent stem cells (hiPSCs)

Popis výsledku anglicky

Astrocytes are specialized glial cell types of the central nervous system (CNS) with remarkably high abundance, morphological and functional diversity. Astrocytes maintain neural metabolic support, synapse regulation, blood-brain barrier integrity and immunological homeostasis through intricate interactions with other cells, including neurons, microglia, pericytes and lymphocytes. Due to their extensive intercellular crosstalks, astrocytes are also implicated in the pathogenesis of CNS disorders, such as ALS (amyotrophic lateral sclerosis), Parkinson's disease and Alzheimer's disease. Despite the critical importance of astrocytes in neurodegeneration and neuroinflammation are recognized, the lack of suitable in vitro systems limits their availability for modeling human brain pathologies. Here, we report the time-efficient, reproducible generation of astrocytes from human induced pluripotent stem cells (hiPSCs). Our hiPSC-derived astrocytes expressed characteristic astrocyte markers, such as GFAP, S100b, ALDH1L1 and AQP4. Furthermore, hiPSC-derived astrocytes displayed spontaneous calcium transients and responded to inflammatory stimuli by the secretion of type A1 and type A2 astrocyte-related cytokines.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

<a href="/cs/project/NU20-09-00437" target="_blank" >NU20-09-00437: Identifikace změn glutamátergních drah specifických pro sporadickou formu Alzheimerovy choroby v lidských neuronech a astrocytech indukovaných z buněk pacientů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS ONE

ISSN

1932-6203

e-ISSN

1932-6203

Svazek periodika

19

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

19

Strana od-do

e0313514

Kód UT WoS článku

001372873500051

EID výsledku v databázi Scopus

2-s2.0-85211063743