Detection and quantification of the canine DNA in meat DNA mixes and meat mixtures by real time PCR and digital droplet PCR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F25%3A00639475" target="_blank" >RIV/67985904:_____/25:00639475 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/25:00142388 RIV/62157124:16270/25:43882467 RIV/00027162:_____/25:N0000039

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0889157525006702?pes=vor&utm_source=clarivate&getft_integrator=clarivate" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0889157525006702?pes=vor&utm_source=clarivate&getft_integrator=clarivate</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jfca.2025.107855" target="_blank" >10.1016/j.jfca.2025.107855</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection and quantification of the canine DNA in meat DNA mixes and meat mixtures by real time PCR and digital droplet PCR

Popis výsledku v původním jazyce

Accurate detection of animal species in meat products is essential for food safety, regulatory compliance, and the prevention of food fraud. This study presents the development and validation of a modified quadruplex quantitative PCR (qPCR) assay and singleplex droplet digital PCR (ddPCR) for the detection and quantification of canine DNA in meat and DNA mixtures. Two- and three-species samples containing canine, pork, and chicken components (0.1 %-100 %) were prepared, including heat-treated variants. A simplified quantification approach using a single 100 % DNA reference diluted to 10 ng/mu L was applied in both methods. Results from qPCR and ddPCR were statistically evaluated using ANOVA, RMSD, and coefficient of variation (CV). Both methods yielded accurate results across most concentration ranges, with no significant differences in calculated percentages. However, qPCR exhibited significantly higher variability than ddPCR, especially at extreme concentrations (<1 % and >99 %). ddPCR showed superior performance in three-species mixtures and yielded lower RMSD values overall. Despite higher variability at detection limits inherent to PCR technology, both methods are suitable for routine quantification of canine DNA in meat products. The study confirms that a single-reference standard is a cost-effective and reliable alternative to full calibration curves for species quantification.

Název v anglickém jazyce

Detection and quantification of the canine DNA in meat DNA mixes and meat mixtures by real time PCR and digital droplet PCR

Popis výsledku anglicky

Accurate detection of animal species in meat products is essential for food safety, regulatory compliance, and the prevention of food fraud. This study presents the development and validation of a modified quadruplex quantitative PCR (qPCR) assay and singleplex droplet digital PCR (ddPCR) for the detection and quantification of canine DNA in meat and DNA mixtures. Two- and three-species samples containing canine, pork, and chicken components (0.1 %-100 %) were prepared, including heat-treated variants. A simplified quantification approach using a single 100 % DNA reference diluted to 10 ng/mu L was applied in both methods. Results from qPCR and ddPCR were statistically evaluated using ANOVA, RMSD, and coefficient of variation (CV). Both methods yielded accurate results across most concentration ranges, with no significant differences in calculated percentages. However, qPCR exhibited significantly higher variability than ddPCR, especially at extreme concentrations (<1 % and >99 %). ddPCR showed superior performance in three-species mixtures and yielded lower RMSD values overall. Despite higher variability at detection limits inherent to PCR technology, both methods are suitable for routine quantification of canine DNA in meat products. The study confirms that a single-reference standard is a cost-effective and reliable alternative to full calibration curves for species quantification.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Food Composition and Analysis

ISSN

0889-1575

e-ISSN

1096-0481

Svazek periodika

146

Číslo periodika v rámci svazku

OCT 25

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

107855

Kód UT WoS článku

001568377000003

EID výsledku v databázi Scopus

2-s2.0-105007061570