Programmed DNA elimination occurs during early gametogenesis in hybridogenetic hybrids from Hexagrammos genus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F25%3A00640399" target="_blank" >RIV/67985904:_____/25:00640399 - isvavai.cz</a>

Výsledek na webu

<a href="https://academic.oup.com/biolreprod/article-abstract/113/3/605/8157711?redirectedFrom=fulltext&login=true" target="_blank" >https://academic.oup.com/biolreprod/article-abstract/113/3/605/8157711?redirectedFrom=fulltext&login=true</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/biolre/ioaf125" target="_blank" >10.1093/biolre/ioaf125</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Programmed DNA elimination occurs during early gametogenesis in hybridogenetic hybrids from Hexagrammos genus

Popis výsledku v původním jazyce

Hybridogenesis is a unique type of reproduction found in hybrids, producing offspring that are partial (hemiclonal) genetic replicas of one parent. Along the southern coast of Hokkaido, Japan, two types of interspecies hybrids have been identified among three Hexagrammos species: H. octogrammus (Hoc), H. otakii (Hot), and H. agrammus (Hag). These hybrids are characterized by exclusively fertile females reproducing via hybridogenesis. During oogenesis of hybrids, the paternal haploid genome (Hag or Hot) is excluded, while only the maternal genome (Hoc) retains and transmits to eggs. This study investigates the mechanism of genome elimination in Hexagrammos hybrids through a comparative analysis of gonadal development in 1-year-old juveniles and adult individuals of hybrids and their parental species. Comparative genomic hybridization on whole-mount gonads from Hoc/Hot and (Hoc/Hag) x Hag hybrids revealed gonocytes containing genomes of both parental species and gonocytes with only Hoc genome, suggesting that genome elimination occurs during early gametogenesis. In Hoc but not in Hot or Hag species, we observed heterochromatin foci enriched with H3K9me3 epigenetic histone modification. Similar foci were found in hybrids, confirming the presence of Hoc genome in their gonocytes. Furthermore, we detected micronuclei in the cytoplasm of gonocytes in parental species and hybrids. Although micronuclei were rare, their frequency was significantly higher in hybrids compared to parental species. Within micronuclei in Hoc/Hot and (Hoc/Hag) x Hag hybrids, we identified Hot and Hag chromosomes or their fragments, respectively. Thus, in Hexagrammos hybrids, genome elimination likely occurs during early gametogenic stages and is accompanied by micronuclei formation.

Název v anglickém jazyce

Programmed DNA elimination occurs during early gametogenesis in hybridogenetic hybrids from Hexagrammos genus

Popis výsledku anglicky

Hybridogenesis is a unique type of reproduction found in hybrids, producing offspring that are partial (hemiclonal) genetic replicas of one parent. Along the southern coast of Hokkaido, Japan, two types of interspecies hybrids have been identified among three Hexagrammos species: H. octogrammus (Hoc), H. otakii (Hot), and H. agrammus (Hag). These hybrids are characterized by exclusively fertile females reproducing via hybridogenesis. During oogenesis of hybrids, the paternal haploid genome (Hag or Hot) is excluded, while only the maternal genome (Hoc) retains and transmits to eggs. This study investigates the mechanism of genome elimination in Hexagrammos hybrids through a comparative analysis of gonadal development in 1-year-old juveniles and adult individuals of hybrids and their parental species. Comparative genomic hybridization on whole-mount gonads from Hoc/Hot and (Hoc/Hag) x Hag hybrids revealed gonocytes containing genomes of both parental species and gonocytes with only Hoc genome, suggesting that genome elimination occurs during early gametogenesis. In Hoc but not in Hot or Hag species, we observed heterochromatin foci enriched with H3K9me3 epigenetic histone modification. Similar foci were found in hybrids, confirming the presence of Hoc genome in their gonocytes. Furthermore, we detected micronuclei in the cytoplasm of gonocytes in parental species and hybrids. Although micronuclei were rare, their frequency was significantly higher in hybrids compared to parental species. Within micronuclei in Hoc/Hot and (Hoc/Hag) x Hag hybrids, we identified Hot and Hag chromosomes or their fragments, respectively. Thus, in Hexagrammos hybrids, genome elimination likely occurs during early gametogenic stages and is accompanied by micronuclei formation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10613 - Zoology

Projekt

<a href="/cs/project/GF23-07028K" target="_blank" >GF23-07028K: Porušení pravidel: modifikované způsoby sexuální reprodukce u obratlovců</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biology of Reproduction

ISSN

0006-3363

e-ISSN

1529-7268

Svazek periodika

113

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

605-614

Kód UT WoS článku

001517882800001

EID výsledku v databázi Scopus

2-s2.0-105016363071