Assessment of Chromosome Congression Defects in Mammalian Oocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F25%3A00644578" target="_blank" >RIV/67985904:_____/25:00644578 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/book/10.1007/978-1-0716-4658-8" target="_blank" >https://link.springer.com/book/10.1007/978-1-0716-4658-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-0716-4658-8_10" target="_blank" >10.1007/978-1-0716-4658-8_10</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Assessment of Chromosome Congression Defects in Mammalian Oocytes

Popis výsledku v původním jazyce

Congression of all chromosomes on the equatorial plane is in mitosis required for metaphase–anaphase transition, and for successful segregation of sister chromatids during anaphase. In contrast to somatic cells, mouse oocytes, which are undergoing the first meiotic division, are capable to undergo metaphase–anaphase transition without congression of all chromosomes. Consequently, oocytes exhibit an increased frequency of chromosome segregation errors, which might lead to aneuploidy. In various experimental conditions, for example, during gene function studies, or during evaluation of the effects of pharmaceutical inhibitors, it might be beneficial to assess chromosome congression in live oocytes. In this chapter, we describe all steps necessary for performing such experiments, including isolation of mouse oocytes, microinjection—free labeling of chromosomes and spindle, the time-lapse recording of chromosome division during meiosis I, using confocal live cell microscopy, and also steps necessary for data analysis.

Název v anglickém jazyce

Assessment of Chromosome Congression Defects in Mammalian Oocytes

Popis výsledku anglicky

Congression of all chromosomes on the equatorial plane is in mitosis required for metaphase–anaphase transition, and for successful segregation of sister chromatids during anaphase. In contrast to somatic cells, mouse oocytes, which are undergoing the first meiotic division, are capable to undergo metaphase–anaphase transition without congression of all chromosomes. Consequently, oocytes exhibit an increased frequency of chromosome segregation errors, which might lead to aneuploidy. In various experimental conditions, for example, during gene function studies, or during evaluation of the effects of pharmaceutical inhibitors, it might be beneficial to assess chromosome congression in live oocytes. In this chapter, we describe all steps necessary for performing such experiments, including isolation of mouse oocytes, microinjection—free labeling of chromosomes and spindle, the time-lapse recording of chromosome division during meiosis I, using confocal live cell microscopy, and also steps necessary for data analysis.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Mammalian Oocyte Development

ISBN

978-1-0716-4657-1

Počet stran výsledku

11

Strana od-do

125-135

Počet stran knihy

214

Název nakladatele

Humana New York

Místo vydání

New York

Kód UT WoS kapitoly

—