Metabolic activity and membrane integrity changes in Microcystis aeruginosa ? new findings on hydrogen peroxide toxicity in cyanobacteria
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F12%3A00379236" target="_blank" >RIV/67985939:_____/12:00379236 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1080/09670262.2012.687144" target="_blank" >http://dx.doi.org/10.1080/09670262.2012.687144</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/09670262.2012.687144" target="_blank" >10.1080/09670262.2012.687144</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Metabolic activity and membrane integrity changes in Microcystis aeruginosa ? new findings on hydrogen peroxide toxicity in cyanobacteria
Popis výsledku v původním jazyce
The aim of our study was to investigate the intracellular toxicity mechanisms of potentially anti-cyanobacterial agent hydrogen peroxide in Microcystis aeruginosa. Metabolic activity and cell membrane integrity were evaluated by flow cytometry in cyanobacteria exposed to the substance tested in the dark or light; the relationships between exposure effects and the kinetics of hydrogen peroxide decomposition were studied. Flow cytometry and chlorophyll a fluorescence measurements suggested that hydrogen peroxide exposure elicits an immediate decline of metabolic activity, and immediate changes of chlorophyll a fluorescence parameters, followed later by an increase in the percentage of membrane-compromised cells. When the concentration of hydrogen peroxide used was lethal, a significant drop in total cell counts was detected. Our study also confirmed that light is the critical factor affecting peroxide decomposition and thus greatly influences its toxicity.
Název v anglickém jazyce
Metabolic activity and membrane integrity changes in Microcystis aeruginosa ? new findings on hydrogen peroxide toxicity in cyanobacteria
Popis výsledku anglicky
The aim of our study was to investigate the intracellular toxicity mechanisms of potentially anti-cyanobacterial agent hydrogen peroxide in Microcystis aeruginosa. Metabolic activity and cell membrane integrity were evaluated by flow cytometry in cyanobacteria exposed to the substance tested in the dark or light; the relationships between exposure effects and the kinetics of hydrogen peroxide decomposition were studied. Flow cytometry and chlorophyll a fluorescence measurements suggested that hydrogen peroxide exposure elicits an immediate decline of metabolic activity, and immediate changes of chlorophyll a fluorescence parameters, followed later by an increase in the percentage of membrane-compromised cells. When the concentration of hydrogen peroxide used was lethal, a significant drop in total cell counts was detected. Our study also confirmed that light is the critical factor affecting peroxide decomposition and thus greatly influences its toxicity.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EF - Botanika
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
European Journal of Phycology
ISSN
0967-0262
e-ISSN
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Svazek periodika
47
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
12
Strana od-do
195-206
Kód UT WoS článku
000308435700001
EID výsledku v databázi Scopus
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