Cross-amplification and multiplexing of SSR markers for Alnus glutinosa and A. incana

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F14%3A00432241" target="_blank" >RIV/67985939:_____/14:00432241 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41330/14:64172

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11295-014-0727-z" target="_blank" >http://dx.doi.org/10.1007/s11295-014-0727-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11295-014-0727-z" target="_blank" >10.1007/s11295-014-0727-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cross-amplification and multiplexing of SSR markers for Alnus glutinosa and A. incana

Popis výsledku v původním jazyce

We investigated 39 previously developed Betula, Alnus, and Corylus simple sequence repeat (SSR) markers for their utility in the cross-generic amplification of two European alder species, i.e., Alnus glutinosa and A. incana. Of these markers, ten loci had successful amplification within Alnus species. Finally, we designed two multiplexes composed of eight and nine loci for A. glutinosa and A. incana, respectively. Multiplexes were tested on 100 samples from five different populations of each species across Europe. The majority of loci had a relatively high genetic diversity, were in Hardy-Weinberg equilibrium, and showed low error rates and low occurrence of null alleles. By comparing sequences of source species and both Alnus species, we concluded that repeat motifs of five of these ten loci differed from those described for the source species. These differences represent mainly the modifications of the original motifs and affected compound or interrupted repeats as well as pure ones.

Název v anglickém jazyce

Cross-amplification and multiplexing of SSR markers for Alnus glutinosa and A. incana

Popis výsledku anglicky

We investigated 39 previously developed Betula, Alnus, and Corylus simple sequence repeat (SSR) markers for their utility in the cross-generic amplification of two European alder species, i.e., Alnus glutinosa and A. incana. Of these markers, ten loci had successful amplification within Alnus species. Finally, we designed two multiplexes composed of eight and nine loci for A. glutinosa and A. incana, respectively. Multiplexes were tested on 100 samples from five different populations of each species across Europe. The majority of loci had a relatively high genetic diversity, were in Hardy-Weinberg equilibrium, and showed low error rates and low occurrence of null alleles. By comparing sequences of source species and both Alnus species, we concluded that repeat motifs of five of these ten loci differed from those described for the source species. These differences represent mainly the modifications of the original motifs and affected compound or interrupted repeats as well as pure ones.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

<a href="/cs/project/GAP504%2F11%2F0402" target="_blank" >GAP504/11/0402: Postglaciální kolonizace druhů Alnus glutinosa a Alnus incana: analýza DNA a fosilních záznamů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Tree Genetics & Genomes

ISSN

1614-2942

e-ISSN

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Svazek periodika

10

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

865-873

Kód UT WoS článku

000339906200008

EID výsledku v databázi Scopus

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