Culture medium mediated aggregation and re-crystallization of silver nanoparticles reduce their toxicity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F18%3A00508571" target="_blank" >RIV/67985939:_____/18:00508571 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/18:73589922 RIV/00216224:14310/18:00106149

Výsledek na webu

<a href="http://hdl.handle.net/11104/0299463" target="_blank" >http://hdl.handle.net/11104/0299463</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.apmt.2018.05.004" target="_blank" >10.1016/j.apmt.2018.05.004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Culture medium mediated aggregation and re-crystallization of silver nanoparticles reduce their toxicity

Popis výsledku v původním jazyce

Due to their wide uses in both domestic and industrial applications it is important to consider possible releases of silver nanoparticles (AgNPs) into the environment and the possible consequences. Thus, the potential toxicity of AgNPs to aquatic organisms has been intensively studied recently. However, culture media composition and stability of AgNPs seriously affect dose-response relationship. In present study the chronic toxicity of AgNPs to Daphnia magna in undiluted and 2-fold diluted media was examined. Both gelatin-stabilized and non-stabilized AgNPs were synthesized and their stability was assessed by transmission electron microscopy and UV/Visible spectroscopy. The half maximal effective concentrations (EC50 values) for D. magna reproduction of non-stabilized AgNPs in undiluted medium, non-stabilized AgNPs in 2-fold diluted medium and AgNPs stabilized with 0.0001% (w/v) gelatin in 2-fold diluted medium were found to be 0.96, 0.32 and 0.16 mg/L, respectively. AgNPs stabilized with 0.005% gelatin did not aggregate and marked toxicity was observed across the AgNP concentration range from 0.001 to 1 mg/L. On the contrary, AgNPs with low stability in media were subjected to aggregation and re-crystallization towards large particles, which reduced their toxicity. Thus, the results demonstrate the importance of rigorous characterization of media composition for robust assessment of AgNPs' toxicological effects.

Název v anglickém jazyce

Culture medium mediated aggregation and re-crystallization of silver nanoparticles reduce their toxicity

Popis výsledku anglicky

Due to their wide uses in both domestic and industrial applications it is important to consider possible releases of silver nanoparticles (AgNPs) into the environment and the possible consequences. Thus, the potential toxicity of AgNPs to aquatic organisms has been intensively studied recently. However, culture media composition and stability of AgNPs seriously affect dose-response relationship. In present study the chronic toxicity of AgNPs to Daphnia magna in undiluted and 2-fold diluted media was examined. Both gelatin-stabilized and non-stabilized AgNPs were synthesized and their stability was assessed by transmission electron microscopy and UV/Visible spectroscopy. The half maximal effective concentrations (EC50 values) for D. magna reproduction of non-stabilized AgNPs in undiluted medium, non-stabilized AgNPs in 2-fold diluted medium and AgNPs stabilized with 0.0001% (w/v) gelatin in 2-fold diluted medium were found to be 0.96, 0.32 and 0.16 mg/L, respectively. AgNPs stabilized with 0.005% gelatin did not aggregate and marked toxicity was observed across the AgNP concentration range from 0.001 to 1 mg/L. On the contrary, AgNPs with low stability in media were subjected to aggregation and re-crystallization towards large particles, which reduced their toxicity. Thus, the results demonstrate the importance of rigorous characterization of media composition for robust assessment of AgNPs' toxicological effects.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20801 - Environmental biotechnology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Materials Today

ISSN

2352-9407

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

SEP 2018

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

198-206

Kód UT WoS článku

000443213700018

EID výsledku v databázi Scopus

2-s2.0-85048470029