DNA barcoding approach fails to discriminate Central European bladderworts (Utricularia, Lentibulariaceae), but provides insights concerning their evolution

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F20%3A00525262" target="_blank" >RIV/67985939:_____/20:00525262 - isvavai.cz</a>

Výsledek na webu

<a href="http://hdl.handle.net/11104/0309444" target="_blank" >http://hdl.handle.net/11104/0309444</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/11263504.2019.1610112" target="_blank" >10.1080/11263504.2019.1610112</a>

Jazyk výsledku

angličtina

Název v původním jazyce

DNA barcoding approach fails to discriminate Central European bladderworts (Utricularia, Lentibulariaceae), but provides insights concerning their evolution

Popis výsledku v původním jazyce

The main features to distinguish the seven native Utricularia species occurring in central Europe are found in flower shape, but flowering identification is often doubtful and uncertain. A recent morphometric work highlighted that there are no univocal reliable extra-floral morphological features allowing a safe identification at species level. Therefore, DNA barcoding approach is attempted here. Molecular analyses were performed to search for DNA barcodes using nuclear ITS (rDNA), plastid (cpDNA) trnL-trnF IGS and rps16 intron sequences. Generally, the barcoding approach failed to discriminate Utricularia species, although it could be of some help in the U. minor aggregate. With few exceptions, U. bremii shows peculiar DNA regions different from U. minor for both plastid markers investigated. However, interesting hypotheses could be derived from the obtained networks, including hybridization events to explain the rise of mostly sterile species, such as U. stygia. This species clusters with the other species of the U. intermedia aggregate in plastid phylogenetic graphs, while it is closely related to species of the U. minor aggregate in ITS phylogenetic graphs.

Název v anglickém jazyce

DNA barcoding approach fails to discriminate Central European bladderworts (Utricularia, Lentibulariaceae), but provides insights concerning their evolution

Popis výsledku anglicky

The main features to distinguish the seven native Utricularia species occurring in central Europe are found in flower shape, but flowering identification is often doubtful and uncertain. A recent morphometric work highlighted that there are no univocal reliable extra-floral morphological features allowing a safe identification at species level. Therefore, DNA barcoding approach is attempted here. Molecular analyses were performed to search for DNA barcodes using nuclear ITS (rDNA), plastid (cpDNA) trnL-trnF IGS and rps16 intron sequences. Generally, the barcoding approach failed to discriminate Utricularia species, although it could be of some help in the U. minor aggregate. With few exceptions, U. bremii shows peculiar DNA regions different from U. minor for both plastid markers investigated. However, interesting hypotheses could be derived from the obtained networks, including hybridization events to explain the rise of mostly sterile species, such as U. stygia. This species clusters with the other species of the U. intermedia aggregate in plastid phylogenetic graphs, while it is closely related to species of the U. minor aggregate in ITS phylogenetic graphs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Biosystems

ISSN

1126-3504

e-ISSN

—

Svazek periodika

154

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

IT - Italská republika

Počet stran výsledku

11

Strana od-do

326-336

Kód UT WoS článku

000471572700001

EID výsledku v databázi Scopus

2-s2.0-85066627928