Structural characteristics and biological effects of exopolysaccharide produced by cyanobacterium Nostoc sp

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F20%3A00532233" target="_blank" >RIV/67985939:_____/20:00532233 - isvavai.cz</a>

Výsledek na webu

<a href="http://hdl.handle.net/11104/0310806" target="_blank" >http://hdl.handle.net/11104/0310806</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2020.05.135" target="_blank" >10.1016/j.ijbiomac.2020.05.135</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Structural characteristics and biological effects of exopolysaccharide produced by cyanobacterium Nostoc sp

Popis výsledku v původním jazyce

Complex structure of cyanobacterium Nostoc sp. exopolysaccharide (EPS), with apparent molecular weight 214 × 103 g/mol, can be deduced from its composition. Chemical and NMR analyses found four dominant sugarmonomers, namely (1→ 4)-linked α-L-arabinopyranose, β-D-glucopyranose, β-D-xylopyranose and (1→ 3)-linked β-D-mannopyranose, two different uronic acids and a lactyl group, with (1→4,6)-linked β-D-glucopyranose as the only branch point suggest a complex structure of this polymer. The dominant uronic acid is α-linked, but it remained unidentified. β-D-Glucuronic acid was present in lower amount. Their position as well as that of lactyl remained undetermined too. Different doses of orally administered EPS in guinea pigs evoked a significant decrease in cough effort and a decrease in airway reactivity. The antitussive efficacy and bronchodilator effect of higher doses of EPS were found to be similar to that of the antitussive drug codeine and the antiasthmatic salbutamol. Without significant cytotoxicity on the RAW264.7 cells, EPS stimulated themacrophage cells to produce pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and prostaglandins (PGs) and nitric oxide (NO) via induction of COX-2 and iNOS expression, respectively, suggesting that this biopolymer potentiates an early innate immune response and can therefore be used as a new immune modulator.

Název v anglickém jazyce

Structural characteristics and biological effects of exopolysaccharide produced by cyanobacterium Nostoc sp

Popis výsledku anglicky

Complex structure of cyanobacterium Nostoc sp. exopolysaccharide (EPS), with apparent molecular weight 214 × 103 g/mol, can be deduced from its composition. Chemical and NMR analyses found four dominant sugarmonomers, namely (1→ 4)-linked α-L-arabinopyranose, β-D-glucopyranose, β-D-xylopyranose and (1→ 3)-linked β-D-mannopyranose, two different uronic acids and a lactyl group, with (1→4,6)-linked β-D-glucopyranose as the only branch point suggest a complex structure of this polymer. The dominant uronic acid is α-linked, but it remained unidentified. β-D-Glucuronic acid was present in lower amount. Their position as well as that of lactyl remained undetermined too. Different doses of orally administered EPS in guinea pigs evoked a significant decrease in cough effort and a decrease in airway reactivity. The antitussive efficacy and bronchodilator effect of higher doses of EPS were found to be similar to that of the antitussive drug codeine and the antiasthmatic salbutamol. Without significant cytotoxicity on the RAW264.7 cells, EPS stimulated themacrophage cells to produce pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and prostaglandins (PGs) and nitric oxide (NO) via induction of COX-2 and iNOS expression, respectively, suggesting that this biopolymer potentiates an early innate immune response and can therefore be used as a new immune modulator.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/TE01020080" target="_blank" >TE01020080: Centrum kompetence pro výzkum biorafinací</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Biological Macromolecules

ISSN

0141-8130

e-ISSN

—

Svazek periodika

160

Číslo periodika v rámci svazku

OCT 1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

364-371

Kód UT WoS článku

000564748800003

EID výsledku v databázi Scopus

2-s2.0-85085972436