Molecular evolution and organization of ribosomal DNA in the hawkweed tribe Hieraciinae (Cichorieae, Asteraceae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F21%3A00543451" target="_blank" >RIV/67985939:_____/21:00543451 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/21:10437663

Výsledek na webu

<a href="https://doi.org/10.3389/fpls.2021.647375" target="_blank" >https://doi.org/10.3389/fpls.2021.647375</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2021.647375" target="_blank" >10.3389/fpls.2021.647375</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular evolution and organization of ribosomal DNA in the hawkweed tribe Hieraciinae (Cichorieae, Asteraceae)

Popis výsledku v původním jazyce

We investigate homogenization and evolution of non-coding multi-copy ribosomal DNA (rDNA) sequences in diploids of the hawkweed subtribe Hieraciinae, comparing results from direct Sanger and Illumina shallow genome sequencing. For the latter, allele phasing is performed, and a software tool is provided that assignes alleles of two different markers for phylogenetic inferences based on concatenated data. In contrast to most other plant groups, 5S-NTS sequences are better homogenized than ITS or ETS sequences. Phylogenetic analyses reveal novel insights in the intricate evolutionary patterns of the group: Ancient hybridization is inferred between genera Hieracium and Hispidella, and sometimes, sequence homogenization occurs in opposite directions. We assess the organization of the rDNA loci corresponding to the respective molecular markers, show the first cytogenetic analysis for genus Andryala, and reconstruct the ancestral state of locus numbers in the Hieraciinae, which corresponds to the ancestral state inferred for plants in general. Different locus numbers, some of them hemizygous, occur in Hieracium, even within populations. Their gains or losses show little correspondence to species relationships. The number of 45S rDNA loci has no detectable influence on sequence homogenization of ITS and ETS sequences contained in the locus, implying rather efficient interlocus concerted evolution.

Název v anglickém jazyce

Molecular evolution and organization of ribosomal DNA in the hawkweed tribe Hieraciinae (Cichorieae, Asteraceae)

Popis výsledku anglicky

We investigate homogenization and evolution of non-coding multi-copy ribosomal DNA (rDNA) sequences in diploids of the hawkweed subtribe Hieraciinae, comparing results from direct Sanger and Illumina shallow genome sequencing. For the latter, allele phasing is performed, and a software tool is provided that assignes alleles of two different markers for phylogenetic inferences based on concatenated data. In contrast to most other plant groups, 5S-NTS sequences are better homogenized than ITS or ETS sequences. Phylogenetic analyses reveal novel insights in the intricate evolutionary patterns of the group: Ancient hybridization is inferred between genera Hieracium and Hispidella, and sometimes, sequence homogenization occurs in opposite directions. We assess the organization of the rDNA loci corresponding to the respective molecular markers, show the first cytogenetic analysis for genus Andryala, and reconstruct the ancestral state of locus numbers in the Hieraciinae, which corresponds to the ancestral state inferred for plants in general. Different locus numbers, some of them hemizygous, occur in Hieracium, even within populations. Their gains or losses show little correspondence to species relationships. The number of 45S rDNA loci has no detectable influence on sequence homogenization of ITS and ETS sequences contained in the locus, implying rather efficient interlocus concerted evolution.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

1664-462X

Svazek periodika

12

Číslo periodika v rámci svazku

Mar 12

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

23

Strana od-do

647375

Kód UT WoS článku

000632855700001

EID výsledku v databázi Scopus

2-s2.0-85103340980