Alternating nuclear DNA content in chrysophytes provides evidence of their isomorphic haploid-diploid life cycle

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F22%3A00562690" target="_blank" >RIV/67985939:_____/22:00562690 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/22:10453010

Výsledek na webu

<a href="https://doi.org/10.1016/j.algal.2022.102707" target="_blank" >https://doi.org/10.1016/j.algal.2022.102707</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.algal.2022.102707" target="_blank" >10.1016/j.algal.2022.102707</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Alternating nuclear DNA content in chrysophytes provides evidence of their isomorphic haploid-diploid life cycle

Popis výsledku v původním jazyce

In many unicellular eukaryotes (protists), the life cycles are still largely unexplored, although this knowledge is key to understanding their biology. We used flow cytometry to detect the often-inconspicuous transitions among life cycle stages in three genera of chrysophyte algae (Chrysosphaerella, Ochromonas, and Synura). We revealed an alternation of two ploidy levels over time in strains maintained in long-term cultivation, both life cycle stages were capable of mitotic growth and shared similar phenotypes. Therefore, chrysophytes have a haploid-diploid life cycle with isomorphic life cycle stages. As far as we know, this is the first report of such life cycle strategy in unicellular algae. Life cycle stages and life stage transitions seem to be synchronized among all cells coexisting within a culture, possibly due to chemical signals. Additionally, ploidy level variation resulting from whole genome doubling was identified in two species of Synura.

Název v anglickém jazyce

Alternating nuclear DNA content in chrysophytes provides evidence of their isomorphic haploid-diploid life cycle

Popis výsledku anglicky

In many unicellular eukaryotes (protists), the life cycles are still largely unexplored, although this knowledge is key to understanding their biology. We used flow cytometry to detect the often-inconspicuous transitions among life cycle stages in three genera of chrysophyte algae (Chrysosphaerella, Ochromonas, and Synura). We revealed an alternation of two ploidy levels over time in strains maintained in long-term cultivation, both life cycle stages were capable of mitotic growth and shared similar phenotypes. Therefore, chrysophytes have a haploid-diploid life cycle with isomorphic life cycle stages. As far as we know, this is the first report of such life cycle strategy in unicellular algae. Life cycle stages and life stage transitions seem to be synchronized among all cells coexisting within a culture, possibly due to chemical signals. Additionally, ploidy level variation resulting from whole genome doubling was identified in two species of Synura.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Algal Research-Biomass Biofuels and Bioproducts

ISSN

2211-9264

e-ISSN

2211-9264

Svazek periodika

64

Číslo periodika v rámci svazku

MAY

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

102707

Kód UT WoS článku

000794241000002

EID výsledku v databázi Scopus

2-s2.0-85128562018