Anxiety in Duckweed–Metabolism and Effect of Diazepam on Lemna minor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F22%3A00563405" target="_blank" >RIV/67985939:_____/22:00563405 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14160/22:00126390

Výsledek na webu

<a href="https://doi.org/10.3390/w14091484" target="_blank" >https://doi.org/10.3390/w14091484</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/w14091484" target="_blank" >10.3390/w14091484</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Anxiety in Duckweed–Metabolism and Effect of Diazepam on Lemna minor

Popis výsledku v původním jazyce

The fate of pharmaceuticals in the human body, from their absorption to excretion is well studied. However, medication often leaves the patient's body in an unchanged or metabolised, yet still active, form. Diazepam and its metabolites, ranging up to 100 mu g/L, have been detected in surface waters worldwide, therefore, the question of its influence on model aquatic plants, such as duckweed (Lemna minor), needs to be addressed. Lemna was cultivated in a Steinberg medium containing diazepam in three concentrations-0.2, 20, and 2000 mu g/L. The activity of superoxide dismutase (SOD) and catalase (CAT), leaf count, mass, and the fluorescence quantum yield of photosynthesis were assessed. The medium was also analysed by LC-MS/MS to determine the concentration of diazepam metabolites. Our results show no negative impact of diazepam on Lemna minor, even in concentrations significantly higher than those that are ecotoxicologically relevant. On the contrary, the influence of diazepam on Lemna suggests growth stimulation and a similarity to the effect diazepam has on the human body. The comparison to the human body may be accurate because gamma-Aminobutyric acid-like (GABA-like) receptors responsible for the effect in humans have also been recently described in plants. Therefore, our results can open an interesting scientific area, indicating that GABA receptors and interference with benzodiazepines are evolutionarily much older than previously anticipated. This could help to answer more questions related to the reaction of aquatic organisms to micropollutants such as psychopharmaceuticals.

Název v anglickém jazyce

Anxiety in Duckweed–Metabolism and Effect of Diazepam on Lemna minor

Popis výsledku anglicky

The fate of pharmaceuticals in the human body, from their absorption to excretion is well studied. However, medication often leaves the patient's body in an unchanged or metabolised, yet still active, form. Diazepam and its metabolites, ranging up to 100 mu g/L, have been detected in surface waters worldwide, therefore, the question of its influence on model aquatic plants, such as duckweed (Lemna minor), needs to be addressed. Lemna was cultivated in a Steinberg medium containing diazepam in three concentrations-0.2, 20, and 2000 mu g/L. The activity of superoxide dismutase (SOD) and catalase (CAT), leaf count, mass, and the fluorescence quantum yield of photosynthesis were assessed. The medium was also analysed by LC-MS/MS to determine the concentration of diazepam metabolites. Our results show no negative impact of diazepam on Lemna minor, even in concentrations significantly higher than those that are ecotoxicologically relevant. On the contrary, the influence of diazepam on Lemna suggests growth stimulation and a similarity to the effect diazepam has on the human body. The comparison to the human body may be accurate because gamma-Aminobutyric acid-like (GABA-like) receptors responsible for the effect in humans have also been recently described in plants. Therefore, our results can open an interesting scientific area, indicating that GABA receptors and interference with benzodiazepines are evolutionarily much older than previously anticipated. This could help to answer more questions related to the reaction of aquatic organisms to micropollutants such as psychopharmaceuticals.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10618 - Ecology

Projekt

<a href="/cs/project/SS01020006" target="_blank" >SS01020006: Hybridní plazmochemická oxidace pro pokročilou dekontaminaci mikropolutantů a dezinfenkci odpadních vod</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Water

ISSN

2073-4441

e-ISSN

2073-4441

Svazek periodika

14

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

12

Strana od-do

1484

Kód UT WoS článku

000796106900001

EID výsledku v databázi Scopus

2-s2.0-85130189332