Metabarcoding of soil environmental DNA to estimate plant diversity globally

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F23%3A00572140" target="_blank" >RIV/67985939:_____/23:00572140 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/23:43906477

Výsledek na webu

<a href="https://doi.org/10.3389/fpls.2023.1106617" target="_blank" >https://doi.org/10.3389/fpls.2023.1106617</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2023.1106617" target="_blank" >10.3389/fpls.2023.1106617</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Metabarcoding of soil environmental DNA to estimate plant diversity globally

Popis výsledku v původním jazyce

Traditional approaches to collecting large-scale biodiversity data pose huge logistical and technical challenges. We assessed how a comparatively simple method based on sequencing a short fragment (P6 loop) of the chloroplast trnL intron from soil environmental DNA (eDNA) characterises global variation in plant diversity and community composition compared with data derived from traditional plant inventory methods. Large-scale plant diversity and community composition patterns revealed by sequencing eDNA from 325 globally distributed soil samples were broadly in accordance with those derived from sources based on empirical (GBIF) or extrapolated plant distribution and diversity data. The success of the eDNA taxonomy assignment, and the overlap of taxon lists between eDNA and GBIF, was greatest at moderate to high latitudes of the northern hemisphere. On average, around half (mean: 51.5% SD 17.6) of local GBIF records were represented in eDNA databases at the species level, depending on the geographic region. Important experimental considerations for plant eDNA studies include using a sampling volume and design to maximise the number of taxa detected and optimising the sequencing depth. However, increasing the coverage of reference sequence databases would yield the most significant improvements in the accuracy of taxonomic assignments made using the P6 loop of the trnL region

Název v anglickém jazyce

Metabarcoding of soil environmental DNA to estimate plant diversity globally

Popis výsledku anglicky

Traditional approaches to collecting large-scale biodiversity data pose huge logistical and technical challenges. We assessed how a comparatively simple method based on sequencing a short fragment (P6 loop) of the chloroplast trnL intron from soil environmental DNA (eDNA) characterises global variation in plant diversity and community composition compared with data derived from traditional plant inventory methods. Large-scale plant diversity and community composition patterns revealed by sequencing eDNA from 325 globally distributed soil samples were broadly in accordance with those derived from sources based on empirical (GBIF) or extrapolated plant distribution and diversity data. The success of the eDNA taxonomy assignment, and the overlap of taxon lists between eDNA and GBIF, was greatest at moderate to high latitudes of the northern hemisphere. On average, around half (mean: 51.5% SD 17.6) of local GBIF records were represented in eDNA databases at the species level, depending on the geographic region. Important experimental considerations for plant eDNA studies include using a sampling volume and design to maximise the number of taxa detected and optimising the sequencing depth. However, increasing the coverage of reference sequence databases would yield the most significant improvements in the accuracy of taxonomic assignments made using the P6 loop of the trnL region

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

1664-462X

Svazek periodika

14

Číslo periodika v rámci svazku

APR 18 2023

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

9

Strana od-do

1106617

Kód UT WoS článku

000980078800001

EID výsledku v databázi Scopus

2-s2.0-85159908348