A fast and reliable LC-MS-MS method for the quantification of saxitoxin in blood plasma samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F24%3A00597609" target="_blank" >RIV/67985939:_____/24:00597609 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/24:00136658

Výsledek na webu

<a href="https://doi.org/10.1093/jat/bkad092" target="_blank" >https://doi.org/10.1093/jat/bkad092</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jat/bkad092" target="_blank" >10.1093/jat/bkad092</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A fast and reliable LC-MS-MS method for the quantification of saxitoxin in blood plasma samples

Popis výsledku v původním jazyce

Saxitoxins (STXs) are potent neurotoxins produced by marine dinoflagellates or freshwater cyanobacteria, known to cause acute and eventually fatal human intoxications, which are classified as paralytic shellfish poisonings (PSPs). Rapid analysis of STXs in blood plasma can be used for a timely diagnosis and confirmation of PSPs. We developed a fast and simple method of STX extraction based on plasma sample acidification and precipitation by acetonitrile, followed by quantification using liquid chromatography-tandem mass spectrometry (LC-MS-MS). Our approach provides the results <= 30 min, with a limit of detection of 2.8 ng/mL and a lower limit of quantification of 5.0 ng/mL. Within-run and between-run precision experiments showed good reproducibility with <= 15% values. Standard curves for calibration were linear with correlation coefficients >= 0.98 across the assay calibration range (5-200 ng/mL). In an interlaboratory analytical exercise, the method was found to be 100% accurate in determining the presence or absence of STX in human plasma specimens, with recovery values of 86-99%. This simple method for STX determination in animal or human plasma can quickly and reliably diagnose STX exposures and confirm suspected PSP cases to facilitate patient treatment or expedite necessary public health or security actions.

Název v anglickém jazyce

A fast and reliable LC-MS-MS method for the quantification of saxitoxin in blood plasma samples

Popis výsledku anglicky

Saxitoxins (STXs) are potent neurotoxins produced by marine dinoflagellates or freshwater cyanobacteria, known to cause acute and eventually fatal human intoxications, which are classified as paralytic shellfish poisonings (PSPs). Rapid analysis of STXs in blood plasma can be used for a timely diagnosis and confirmation of PSPs. We developed a fast and simple method of STX extraction based on plasma sample acidification and precipitation by acetonitrile, followed by quantification using liquid chromatography-tandem mass spectrometry (LC-MS-MS). Our approach provides the results <= 30 min, with a limit of detection of 2.8 ng/mL and a lower limit of quantification of 5.0 ng/mL. Within-run and between-run precision experiments showed good reproducibility with <= 15% values. Standard curves for calibration were linear with correlation coefficients >= 0.98 across the assay calibration range (5-200 ng/mL). In an interlaboratory analytical exercise, the method was found to be 100% accurate in determining the presence or absence of STX in human plasma specimens, with recovery values of 86-99%. This simple method for STX determination in animal or human plasma can quickly and reliably diagnose STX exposures and confirm suspected PSP cases to facilitate patient treatment or expedite necessary public health or security actions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Analytical Toxicology

ISSN

0146-4760

e-ISSN

1945-2403

Svazek periodika

48

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

119-125

Kód UT WoS článku

001146868300001

EID výsledku v databázi Scopus

2-s2.0-85186540329