Circular dichroism spectroscopy reveals invariant conformation of guanine runs in DNA.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F02%3A17023011" target="_blank" >RIV/68081707:_____/02:17023011 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Circular dichroism spectroscopy reveals invariant conformation of guanine runs in DNA.

Popis výsledku v původním jazyce

We demonstrate that the characteristic circular dichroism (CD) features of the parallel-stranded DNA tetraplex of d(G(4)), especially the strong band at 260 nm, are characteristic for the B and A forms of the antiparallel duplex of d(C(4)G(4)). Hence, this band evidently originates from intrastrand guanine-guanine stacking, which is therefore very similar in the duplex and tetraplex DNA. In addition, the same type of the CD spectrum is provided by the ordered single strand of d(GA)(10). This observationsuggests that the ordered single strand of d(GA)(10) is stabilized by a core of guanines stacked like in the parallel tetraplex. This view is used to start the modeling of the molecular structure of the ordered d(GA)(10) single strand. Our studies suggest that guanine itself is strong enough to stabilize various secondary structures of DNA, which is a property relevant to thinking about the origin and evolution of molecular replicators.

Název v anglickém jazyce

Circular dichroism spectroscopy reveals invariant conformation of guanine runs in DNA.

Popis výsledku anglicky

We demonstrate that the characteristic circular dichroism (CD) features of the parallel-stranded DNA tetraplex of d(G(4)), especially the strong band at 260 nm, are characteristic for the B and A forms of the antiparallel duplex of d(C(4)G(4)). Hence, this band evidently originates from intrastrand guanine-guanine stacking, which is therefore very similar in the duplex and tetraplex DNA. In addition, the same type of the CD spectrum is provided by the ordered single strand of d(GA)(10). This observationsuggests that the ordered single strand of d(GA)(10) is stabilized by a core of guanines stacked like in the parallel tetraplex. This view is used to start the modeling of the molecular structure of the ordered d(GA)(10) single strand. Our studies suggest that guanine itself is strong enough to stabilize various secondary structures of DNA, which is a property relevant to thinking about the origin and evolution of molecular replicators.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biopolymers

ISSN

0006-3525

e-ISSN

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Svazek periodika

67

Číslo periodika v rámci svazku

4/5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

3

Strana od-do

275-277

Kód UT WoS článku

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EID výsledku v databázi Scopus

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