The X Chromosome Is Necessary for Somatic Development in the Dioecious Silene latifolia: Cytogenetic and Molecular Evidence and Sequencing of a Haploid Genome

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F14%3A00436672" target="_blank" >RIV/68081707:_____/14:00436672 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/14:00074437 RIV/61389030:_____/14:00436672

Výsledek na webu

<a href="http://dx.doi.org/10.1159/000363431" target="_blank" >http://dx.doi.org/10.1159/000363431</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1159/000363431" target="_blank" >10.1159/000363431</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The X Chromosome Is Necessary for Somatic Development in the Dioecious Silene latifolia: Cytogenetic and Molecular Evidence and Sequencing of a Haploid Genome

Popis výsledku v původním jazyce

Silene latifolia (or white campion) possesses a well-established sex determination system with a dominant Y chromosome in males (the mammalian type). The heteromorphic sex chromosomes X and Y in S. latifolia largely stopped recombination; thus, we can expect a gradual genetic degeneration of the Y chromosome. It is well proven that neither diploid nor polyploid S. latifolia sporophytes can survive without at least one X, so the only life stage possessing the Y as the sole sex chromosome is the male gametophyte (pollen tube), while the female gametophyte seems to be X-dependent. Previous studies on anther-derived plants of this species showed that the obtained plants (largely haploid or dihaploid) were phenotypically and cytologically female. In this paper, we provide molecular evidence for the inviability of plants lacking the X chromosome. Using sex-specific PCR primers, we show that all plantlets and plants derived from anther cultures are female. In studying anther-derived diploid f

Název v anglickém jazyce

The X Chromosome Is Necessary for Somatic Development in the Dioecious Silene latifolia: Cytogenetic and Molecular Evidence and Sequencing of a Haploid Genome

Popis výsledku anglicky

Silene latifolia (or white campion) possesses a well-established sex determination system with a dominant Y chromosome in males (the mammalian type). The heteromorphic sex chromosomes X and Y in S. latifolia largely stopped recombination; thus, we can expect a gradual genetic degeneration of the Y chromosome. It is well proven that neither diploid nor polyploid S. latifolia sporophytes can survive without at least one X, so the only life stage possessing the Y as the sole sex chromosome is the male gametophyte (pollen tube), while the female gametophyte seems to be X-dependent. Previous studies on anther-derived plants of this species showed that the obtained plants (largely haploid or dihaploid) were phenotypically and cytologically female. In this paper, we provide molecular evidence for the inviability of plants lacking the X chromosome. Using sex-specific PCR primers, we show that all plantlets and plants derived from anther cultures are female. In studying anther-derived diploid f

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cytogenetics and Genome Research

ISSN

1424-8581

e-ISSN

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Svazek periodika

143

Číslo periodika v rámci svazku

1-3

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

8

Strana od-do

96-103

Kód UT WoS článku

000342614100010

EID výsledku v databázi Scopus

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