Electrochemical behavior of anthraquinone- and nitrophenyl-labeled deoxynucleoside triphosphates: a contribution to development of multipotential redox labeling of DNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00446294" target="_blank" >RIV/68081707:_____/15:00446294 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/15:00081382

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00706-015-1435-6" target="_blank" >http://dx.doi.org/10.1007/s00706-015-1435-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-015-1435-6" target="_blank" >10.1007/s00706-015-1435-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical behavior of anthraquinone- and nitrophenyl-labeled deoxynucleoside triphosphates: a contribution to development of multipotential redox labeling of DNA

Popis výsledku v původním jazyce

Electrochemical properties of base-modified cytosine or 7-deazaadenine nucleoside triphosphates (dNTPs) bearing electrochemically active anthraquinone or 3-nitrophenyl moieties were studied using cyclic voltammetry with the hanging mercury drop electrode. The anthraquinone moiety in the dNTPs gives well-pronounced reversible quinone/hydroquinone redox signals around -0.40 V (against Ag|AgCl|3M KCl reference electrode), while the nitro group in 3-nitrophenyl exhibits irreversible reduction to hydroxylamine around -0.45 V that can be reversibly oxidized to corresponding nitroso compound close to 0.0 V. Both anthraquinone and hydroxylamine redox groups can be selectively switched off by further electrochemical transformation, depending on negative potential applied and composition of the background electrolyte. Results of this study suggest that both nucleobase and the conjugate label moiety influence remarkably the adsorbability and/or intermolecular interactions taking part at the elect

Název v anglickém jazyce

Electrochemical behavior of anthraquinone- and nitrophenyl-labeled deoxynucleoside triphosphates: a contribution to development of multipotential redox labeling of DNA

Popis výsledku anglicky

Electrochemical properties of base-modified cytosine or 7-deazaadenine nucleoside triphosphates (dNTPs) bearing electrochemically active anthraquinone or 3-nitrophenyl moieties were studied using cyclic voltammetry with the hanging mercury drop electrode. The anthraquinone moiety in the dNTPs gives well-pronounced reversible quinone/hydroquinone redox signals around -0.40 V (against Ag|AgCl|3M KCl reference electrode), while the nitro group in 3-nitrophenyl exhibits irreversible reduction to hydroxylamine around -0.45 V that can be reversibly oxidized to corresponding nitroso compound close to 0.0 V. Both anthraquinone and hydroxylamine redox groups can be selectively switched off by further electrochemical transformation, depending on negative potential applied and composition of the background electrolyte. Results of this study suggest that both nucleobase and the conjugate label moiety influence remarkably the adsorbability and/or intermolecular interactions taking part at the elect

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte fur Chemie

ISSN

0026-9247

e-ISSN

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Svazek periodika

146

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

9

Strana od-do

839-847

Kód UT WoS článku

000353219700014

EID výsledku v databázi Scopus

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