Silenced rRNA genes are activated and substitute for partially eliminated active homeologs in the recently formed allotetraploid, Tragopogon mirus (Asteraceae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00446339" target="_blank" >RIV/68081707:_____/15:00446339 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1038/hdy.2014.111" target="_blank" >http://dx.doi.org/10.1038/hdy.2014.111</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/hdy.2014.111" target="_blank" >10.1038/hdy.2014.111</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Silenced rRNA genes are activated and substitute for partially eliminated active homeologs in the recently formed allotetraploid, Tragopogon mirus (Asteraceae)

Popis výsledku v původním jazyce

To study the relationship between uniparental rDNA (encoding 18S, 5.8S and 26S ribosomal RNA) silencing (nucleolar dominance) and rRNA gene dosage, we studied a recently emerged (within the last 80 years) allotetraploid Tragopogon mirus (2n= 24), formedfrom the diploid progenitors T. dubius (2n= 12, D-genome donor) and T. porrifolius (2n= 12, P-genome donor). Here, we used molecular, cytogenetic and genomic approaches to analyse rRNA gene activity in two sibling T. mirus plants (33A and 33B) with widely different rRNA gene dosages. Plant 33B had similar to 400 rRNA genes at the D-genome locus, which is typical for T. mirus, accounting for similar to 25% of total rDNA. We observed characteristic expression dominance of T. dubius-origin genes in all organs. Its sister plant 33A harboured a homozygous macrodeletion that reduced the number of T. dubius-origin genes to about 70 copies (similar to 4% of total rDNA). It showed biparental rDNA expression in root, flower and callus, but not in

Název v anglickém jazyce

Silenced rRNA genes are activated and substitute for partially eliminated active homeologs in the recently formed allotetraploid, Tragopogon mirus (Asteraceae)

Popis výsledku anglicky

To study the relationship between uniparental rDNA (encoding 18S, 5.8S and 26S ribosomal RNA) silencing (nucleolar dominance) and rRNA gene dosage, we studied a recently emerged (within the last 80 years) allotetraploid Tragopogon mirus (2n= 24), formedfrom the diploid progenitors T. dubius (2n= 12, D-genome donor) and T. porrifolius (2n= 12, P-genome donor). Here, we used molecular, cytogenetic and genomic approaches to analyse rRNA gene activity in two sibling T. mirus plants (33A and 33B) with widely different rRNA gene dosages. Plant 33B had similar to 400 rRNA genes at the D-genome locus, which is typical for T. mirus, accounting for similar to 25% of total rDNA. We observed characteristic expression dominance of T. dubius-origin genes in all organs. Its sister plant 33A harboured a homozygous macrodeletion that reduced the number of T. dubius-origin genes to about 70 copies (similar to 4% of total rDNA). It showed biparental rDNA expression in root, flower and callus, but not in

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Heredity

ISSN

0018-067X

e-ISSN

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Svazek periodika

114

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

356-365

Kód UT WoS článku

000349671000013

EID výsledku v databázi Scopus

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