Iron(II) Supramolecular Helicates Condense Plasmid DNA and Inhibit Vital DNA-Related Enzymatic Activities

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00447649" target="_blank" >RIV/68081707:_____/15:00447649 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/chem.201501307" target="_blank" >http://dx.doi.org/10.1002/chem.201501307</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/chem.201501307" target="_blank" >10.1002/chem.201501307</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Iron(II) Supramolecular Helicates Condense Plasmid DNA and Inhibit Vital DNA-Related Enzymatic Activities

Popis výsledku v původním jazyce

The dinuclear iron(II) supramolecular helicates [Fe2L3]Cl-4 (L= C25H20N4) bind to DNA through noncovalent (i. e., hydrogen-bonding, electrostatic) interactions and exhibit antimicrobial and anticancer effects. In this study, we show that the helicates condense plasmid DNA with a much higher potency than conventional DNA-condensing agents. Notably, molecules of DNA in the presence of the M enantiomer of [Fe2L]Cl-4 do not form intermolecular aggregates typically formed by other condensing agents, such asspermidine or spermine. The helicates inhibit the activity of several DNA-processing enzymes, such as RNA polymerase, DNA topoisomerase I, deoxyribonuclease I, and site-specific restriction endonucleases. However, the results also indicate that the DNA condensation induced by the helicates does not play a crucial role in these inhibition reactions. The mechanisms for the inhibitory effects of [Fe2L3]Cl-4 helicates on DNA-related enzymatic activities have been proposed.

Název v anglickém jazyce

Iron(II) Supramolecular Helicates Condense Plasmid DNA and Inhibit Vital DNA-Related Enzymatic Activities

Popis výsledku anglicky

The dinuclear iron(II) supramolecular helicates [Fe2L3]Cl-4 (L= C25H20N4) bind to DNA through noncovalent (i. e., hydrogen-bonding, electrostatic) interactions and exhibit antimicrobial and anticancer effects. In this study, we show that the helicates condense plasmid DNA with a much higher potency than conventional DNA-condensing agents. Notably, molecules of DNA in the presence of the M enantiomer of [Fe2L]Cl-4 do not form intermolecular aggregates typically formed by other condensing agents, such asspermidine or spermine. The helicates inhibit the activity of several DNA-processing enzymes, such as RNA polymerase, DNA topoisomerase I, deoxyribonuclease I, and site-specific restriction endonucleases. However, the results also indicate that the DNA condensation induced by the helicates does not play a crucial role in these inhibition reactions. The mechanisms for the inhibitory effects of [Fe2L3]Cl-4 helicates on DNA-related enzymatic activities have been proposed.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/GA13-08273S" target="_blank" >GA13-08273S: Biofyzikální analýza kondenzace DNA. Efekt nových organokovových sloučenin s biologickou aktivitou.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemistry - A European Journal

ISSN

0947-6539

e-ISSN

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Svazek periodika

21

Číslo periodika v rámci svazku

31

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

11189-11195

Kód UT WoS článku

000358216200034

EID výsledku v databázi Scopus

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