Electrochemical and circular dichroism spectroscopic evidence of two types of interaction between [Ru(NH3)(6)](3+) and an elongated thrombin binding aptamer G-quadruplex

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00455887" target="_blank" >RIV/68081707:_____/15:00455887 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.electacta.2015.05.023" target="_blank" >http://dx.doi.org/10.1016/j.electacta.2015.05.023</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.electacta.2015.05.023" target="_blank" >10.1016/j.electacta.2015.05.023</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical and circular dichroism spectroscopic evidence of two types of interaction between [Ru(NH3)(6)](3+) and an elongated thrombin binding aptamer G-quadruplex

Popis výsledku v původním jazyce

In the field of electrochemical biosensing based on DNA probes immobilised at electrode surfaces the [Ru(NH3)(6)](3+) redox marker is commonly used for quantitative characterisation and/or detection purposes. Although it is generally assumed that the interactions between the hexaammineruthenium(III) cation and DNA are solely of electrostatic nature, and thus non-specific in essence, it is shown herein that this is not the case for a sequence containing a G-quadruplex part. The present work investigatesthe interactions of [Ru(NH3)(6)](3+) with an oligonucleotide (21-mer) consisting of the thrombin binding aptamer sequence (a G-quadruplex) elongated by a 5'-overhang of six nucleotides. Combining circular dichroism and electrochemical analyses by differential pulse voltammetry, alternating current voltammetry and cyclic voltammetry, it is demonstrated that the overall interaction stoichiometry (number of [Ru(NH3)(6)](3+) bound to one DNA strand) is lower than the 7 [Ru(NH3)(6)](3+) per s

Název v anglickém jazyce

Electrochemical and circular dichroism spectroscopic evidence of two types of interaction between [Ru(NH3)(6)](3+) and an elongated thrombin binding aptamer G-quadruplex

Popis výsledku anglicky

In the field of electrochemical biosensing based on DNA probes immobilised at electrode surfaces the [Ru(NH3)(6)](3+) redox marker is commonly used for quantitative characterisation and/or detection purposes. Although it is generally assumed that the interactions between the hexaammineruthenium(III) cation and DNA are solely of electrostatic nature, and thus non-specific in essence, it is shown herein that this is not the case for a sequence containing a G-quadruplex part. The present work investigatesthe interactions of [Ru(NH3)(6)](3+) with an oligonucleotide (21-mer) consisting of the thrombin binding aptamer sequence (a G-quadruplex) elongated by a 5'-overhang of six nucleotides. Combining circular dichroism and electrochemical analyses by differential pulse voltammetry, alternating current voltammetry and cyclic voltammetry, it is demonstrated that the overall interaction stoichiometry (number of [Ru(NH3)(6)](3+) bound to one DNA strand) is lower than the 7 [Ru(NH3)(6)](3+) per s

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrochimica acta

ISSN

0013-4686

e-ISSN

—

Svazek periodika

179

Číslo periodika v rámci svazku

OCT 2015

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

84-92

Kód UT WoS článku

000362292200012

EID výsledku v databázi Scopus

—