Inhibition of beta-catenin signalling promotes DNA damage elicited by benzo[a]pyrene in a model of human colon cancer cells via CYP1 deregulation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00456480" target="_blank" >RIV/68081707:_____/15:00456480 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378041:_____/15:00456480 RIV/00027162:_____/15:#0001341 RIV/00216224:14310/15:00095774

Výsledek na webu

<a href="http://dx.doi.org/10.1093/mutage/gev019" target="_blank" >http://dx.doi.org/10.1093/mutage/gev019</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/mutage/gev019" target="_blank" >10.1093/mutage/gev019</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Inhibition of beta-catenin signalling promotes DNA damage elicited by benzo[a]pyrene in a model of human colon cancer cells via CYP1 deregulation

Popis výsledku v původním jazyce

Deregulation of Wnt/beta-catenin signalling plays an important role in the pathogenesis of colorectal cancer. Interestingly, this pathway has been recently implicated in transcriptional control of cytochrome P450 (CYP) family 1 enzymes, which are responsible for bioactivation of a number of dietary carcinogens. In the present study, we investigated the impact of inhibition of Wnt/beta-catenin pathway on metabolism and genotoxicity of benzo[a]pyrene (BaP), a highly mutagenic polycyclic aromatic hydrocarbon and an efficient ligand of the aryl hydrocarbon receptor, which is known as a primary regulator of CYP1 expression, in cellular models derived from colorectal tumours. We observed that a synthetic inhibitor of beta-catenin, JW74, significantly increased formation of BaP-induced DNA adducts in both colorectal adenoma and carcinoma-derived cell lines. Using the short interfering RNA (siRNA) targeting beta-catenin, we then found that beta-catenin knockdown in HCT116 colon carcinoma cells

Název v anglickém jazyce

Inhibition of beta-catenin signalling promotes DNA damage elicited by benzo[a]pyrene in a model of human colon cancer cells via CYP1 deregulation

Popis výsledku anglicky

Deregulation of Wnt/beta-catenin signalling plays an important role in the pathogenesis of colorectal cancer. Interestingly, this pathway has been recently implicated in transcriptional control of cytochrome P450 (CYP) family 1 enzymes, which are responsible for bioactivation of a number of dietary carcinogens. In the present study, we investigated the impact of inhibition of Wnt/beta-catenin pathway on metabolism and genotoxicity of benzo[a]pyrene (BaP), a highly mutagenic polycyclic aromatic hydrocarbon and an efficient ligand of the aryl hydrocarbon receptor, which is known as a primary regulator of CYP1 expression, in cellular models derived from colorectal tumours. We observed that a synthetic inhibitor of beta-catenin, JW74, significantly increased formation of BaP-induced DNA adducts in both colorectal adenoma and carcinoma-derived cell lines. Using the short interfering RNA (siRNA) targeting beta-catenin, we then found that beta-catenin knockdown in HCT116 colon carcinoma cells

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/GA13-09766S" target="_blank" >GA13-09766S: Lipidové složky výživy jako modulátory metabolismu a toxicity xenobiotik v epiteliálních buňkách tlustého střeva</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mutagenesis

ISSN

0267-8357

e-ISSN

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Svazek periodika

30

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

565-576

Kód UT WoS článku

000357885900012

EID výsledku v databázi Scopus

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