Label-free electrochemical analysis of chitosan and glucosamine-containing oligosaccharides

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F16%3A00455881" target="_blank" >RIV/68081707:_____/16:00455881 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.electacta.2015.11.072" target="_blank" >http://dx.doi.org/10.1016/j.electacta.2015.11.072</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.electacta.2015.11.072" target="_blank" >10.1016/j.electacta.2015.11.072</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Label-free electrochemical analysis of chitosan and glucosamine-containing oligosaccharides

Popis výsledku v původním jazyce

Recently we have shown that, in difference to most of polysaccharides, which are considered as electroinactive, chitosan samples containing both glucosamine (GlcN) and N-acetylated glucosamine (GlcNAc) residues are involved in the catalytic hydrogen evolution reaction (CHER) at mercury electrodes, and can be determined by voltammetric or chronopotentiometric stripping. Here we show that polysaccharides and oligosaccharides composed of GlcN, but not those containing only GlcNAc residues, take part in CHER, allowing their easy label-free electrochemical detection. GlcNAc-containing oligosaccharides can be easily transformed into electroactive species by deacetylation using hot KOH treatment. This procedure can be applied also to electroinactive GlcNAc-containing glycans, frequently occurring in glycoproteins, allowing their electrochemical determination at submicromolar level. (C) 2015 Elsevier Ltd. All rights reserved.

Název v anglickém jazyce

Label-free electrochemical analysis of chitosan and glucosamine-containing oligosaccharides

Popis výsledku anglicky

Recently we have shown that, in difference to most of polysaccharides, which are considered as electroinactive, chitosan samples containing both glucosamine (GlcN) and N-acetylated glucosamine (GlcNAc) residues are involved in the catalytic hydrogen evolution reaction (CHER) at mercury electrodes, and can be determined by voltammetric or chronopotentiometric stripping. Here we show that polysaccharides and oligosaccharides composed of GlcN, but not those containing only GlcNAc residues, take part in CHER, allowing their easy label-free electrochemical detection. GlcNAc-containing oligosaccharides can be easily transformed into electroactive species by deacetylation using hot KOH treatment. This procedure can be applied also to electroinactive GlcNAc-containing glycans, frequently occurring in glycoproteins, allowing their electrochemical determination at submicromolar level. (C) 2015 Elsevier Ltd. All rights reserved.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/GA15-15479S" target="_blank" >GA15-15479S: Nové nástroje pro výzkum a diagnostiku nemocí. Mikrofluidické reaktory a elektrochemie pro analýzu proteinů a jejich glykosylaci.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrochimica acta

ISSN

0013-4686

e-ISSN

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Svazek periodika

187

Číslo periodika v rámci svazku

JAN 1 2016

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

375-380

Kód UT WoS článku

000367235600044

EID výsledku v databázi Scopus

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