The dual role of asporin in breast cancer progression
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F16%3A00471962" target="_blank" >RIV/68081707:_____/16:00471962 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00159816:_____/16:00065293 RIV/00216208:11150/16:10332639 RIV/61989592:15110/16:33160240 RIV/00209805:_____/16:N0000090 RIV/00216224:14310/16:00100455
Výsledek na webu
<a href="http://dx.doi.org/10.18632/oncotarget.10471" target="_blank" >http://dx.doi.org/10.18632/oncotarget.10471</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.18632/oncotarget.10471" target="_blank" >10.18632/oncotarget.10471</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The dual role of asporin in breast cancer progression
Popis výsledku v původním jazyce
Asporin has been reported as a tumor suppressor in breast cancer, while asporin-activated invasion has been described in gastric cancer. According to our in silico search, high asporin expresion associates with significantly better relapse free survival (RFS) in patients with low-grade tumors but RFS is significantly worse in patients with grade 3 tumors. In line with other studies, we have confirmed asporin expression by RNA scope in situ hybridization in cancer associated fibroblasts. We have also found asporin expression in the Hs578T breast cancer cell line which we confirmed by quantitative RT-PCR and western blotting. From multiple testing, we found that asporin can be downregulated by bone morphogenetic protein 4 while upregulation may be facilited by serum-free cultivation or by three dimensional growth in stiff Alvetex scaffold. Downregulation by shRNA inhibited invasion of Hs578T as well as of CAFs and T47D cells. Invasion of asporin-negative MDA-MB-231 and BT549 breast cancer cells through collagen type I was enhanced by recombinant asporin. Besides other investigations, large scale analysis of aspartic acid repeat polymorphism will be needed for clarification of the asporin dual role in progression of breast cancer.
Název v anglickém jazyce
The dual role of asporin in breast cancer progression
Popis výsledku anglicky
Asporin has been reported as a tumor suppressor in breast cancer, while asporin-activated invasion has been described in gastric cancer. According to our in silico search, high asporin expresion associates with significantly better relapse free survival (RFS) in patients with low-grade tumors but RFS is significantly worse in patients with grade 3 tumors. In line with other studies, we have confirmed asporin expression by RNA scope in situ hybridization in cancer associated fibroblasts. We have also found asporin expression in the Hs578T breast cancer cell line which we confirmed by quantitative RT-PCR and western blotting. From multiple testing, we found that asporin can be downregulated by bone morphogenetic protein 4 while upregulation may be facilited by serum-free cultivation or by three dimensional growth in stiff Alvetex scaffold. Downregulation by shRNA inhibited invasion of Hs578T as well as of CAFs and T47D cells. Invasion of asporin-negative MDA-MB-231 and BT549 breast cancer cells through collagen type I was enhanced by recombinant asporin. Besides other investigations, large scale analysis of aspartic acid repeat polymorphism will be needed for clarification of the asporin dual role in progression of breast cancer.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
BO - Biofyzika
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
OncoTarget
ISSN
1949-2553
e-ISSN
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Svazek periodika
7
Číslo periodika v rámci svazku
32
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
16
Strana od-do
52045-52060
Kód UT WoS článku
000385429100101
EID výsledku v databázi Scopus
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