Label-free detection of canonical DNA bases, uracil and 5-methylcytosine in DNA oligonucleotides using linear sweep voltammetry at a pyrolytic graphite electrode

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F17%3A00485557" target="_blank" >RIV/68081707:_____/17:00485557 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.elecom.2017.07.013" target="_blank" >http://dx.doi.org/10.1016/j.elecom.2017.07.013</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.elecom.2017.07.013" target="_blank" >10.1016/j.elecom.2017.07.013</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Label-free detection of canonical DNA bases, uracil and 5-methylcytosine in DNA oligonucleotides using linear sweep voltammetry at a pyrolytic graphite electrode

Popis výsledku v původním jazyce

An innovative approach to label-free voltammetric analysis of DNA at a pyrolytic graphite electrode (PGE) within a broad range of potentials (from2.0 to + 1.6 V) in an acetate buffer (pH 5) is presented. Using specifically designed DNA nonamers, we demonstrate not only anodic oxidation, but for the first time also cathodic reduction of nucleobases at the PGE. In addition, products of irreversible oxidation/reduction of the parent bases are shown to yield analytically useful, base-specific cathodic/anodic signals, making it possible to distinguish between the canonical bases (adenine, cytosine, guanine and thymine), uracil (U) and 5-methylcytosine (mC) in DNA. Furthermore, selective electrochemical switching off of the redox signals specific to certain nucleobases is presented as a way to resolve overlapping signals. Similarly, newly reported signals corresponding to electrochemically transformed bases can be switched on under specific conditions. This approach can be utilized for fast and facile simultaneous label-free analysis of bases in DNA, including mC and U, and to uncover overlapping signals. This significantly extends the possible applications of PGE in DNA research and (bio) sensor development.

Název v anglickém jazyce

Label-free detection of canonical DNA bases, uracil and 5-methylcytosine in DNA oligonucleotides using linear sweep voltammetry at a pyrolytic graphite electrode

Popis výsledku anglicky

An innovative approach to label-free voltammetric analysis of DNA at a pyrolytic graphite electrode (PGE) within a broad range of potentials (from2.0 to + 1.6 V) in an acetate buffer (pH 5) is presented. Using specifically designed DNA nonamers, we demonstrate not only anodic oxidation, but for the first time also cathodic reduction of nucleobases at the PGE. In addition, products of irreversible oxidation/reduction of the parent bases are shown to yield analytically useful, base-specific cathodic/anodic signals, making it possible to distinguish between the canonical bases (adenine, cytosine, guanine and thymine), uracil (U) and 5-methylcytosine (mC) in DNA. Furthermore, selective electrochemical switching off of the redox signals specific to certain nucleobases is presented as a way to resolve overlapping signals. Similarly, newly reported signals corresponding to electrochemically transformed bases can be switched on under specific conditions. This approach can be utilized for fast and facile simultaneous label-free analysis of bases in DNA, including mC and U, and to uncover overlapping signals. This significantly extends the possible applications of PGE in DNA research and (bio) sensor development.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrochemistry Communications

ISSN

1388-2481

e-ISSN

—

Svazek periodika

82

Číslo periodika v rámci svazku

SEP2017

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

34-38

Kód UT WoS článku

000408359600008

EID výsledku v databázi Scopus

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