Lable-free electrochemical analysis of biomacromolecules

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F17%3A00486104" target="_blank" >RIV/68081707:_____/17:00486104 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.apmt.2017.08.011" target="_blank" >http://dx.doi.org/10.1016/j.apmt.2017.08.011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.apmt.2017.08.011" target="_blank" >10.1016/j.apmt.2017.08.011</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Lable-free electrochemical analysis of biomacromolecules

Popis výsledku v původním jazyce

Recent progress in label-free electrochemical analysis of biomacromolecules, such as proteins, nucleicacids and carbohydrates is reviewed. Since the 1970s electrochemical analysis of proteins focused on non-protein redox-active components of a relatively small group of conjugated proteins. In the recent decade, the ability of practically of all proteins to catalyze hydrogen evolution at mercury-containing electrodes was utilized for development of the protein structure-sensitive analysis. Some amino acid residues, such as arginine, lysine and cysteine contribute to the catalytic hydrogen evolution reaction (CHER) at neutral pH yielding protein reduction signals at highly negative potentials. It was found that native proteins do not lose their folded structure when adsorbed at mercury electrode close to the potential of zero charge. Surface-attached proteins get however denatured due to the electric field effects during their prolonged exposure to negative potentials. Using the constant current chronopotentiometric stripping it was possible to limit the exposure time to milliseconds preventing protein denaturation. The method was utilized in detection of changes in protein structures due to mutation, chemical modification, aggregation, damage by environmental agents, as well as to studies of poorly soluble membrane proteins, DNA-protein and protein-protein interactions, etc. Application of voltammetric methods, such as fast scan CV and normal pulse voltammetry showed smaller sensitivity to tiny changes in protein structures. Recently CHER was found also in some polysaccharides such as chitosan and in NH2 group-containing glycans. Very recent development in electrochemical analysis of DNA and RNA was briefly summarized. (C) 2017 Elsevier Ltd. All rights reserved.

Název v anglickém jazyce

Lable-free electrochemical analysis of biomacromolecules

Popis výsledku anglicky

Recent progress in label-free electrochemical analysis of biomacromolecules, such as proteins, nucleicacids and carbohydrates is reviewed. Since the 1970s electrochemical analysis of proteins focused on non-protein redox-active components of a relatively small group of conjugated proteins. In the recent decade, the ability of practically of all proteins to catalyze hydrogen evolution at mercury-containing electrodes was utilized for development of the protein structure-sensitive analysis. Some amino acid residues, such as arginine, lysine and cysteine contribute to the catalytic hydrogen evolution reaction (CHER) at neutral pH yielding protein reduction signals at highly negative potentials. It was found that native proteins do not lose their folded structure when adsorbed at mercury electrode close to the potential of zero charge. Surface-attached proteins get however denatured due to the electric field effects during their prolonged exposure to negative potentials. Using the constant current chronopotentiometric stripping it was possible to limit the exposure time to milliseconds preventing protein denaturation. The method was utilized in detection of changes in protein structures due to mutation, chemical modification, aggregation, damage by environmental agents, as well as to studies of poorly soluble membrane proteins, DNA-protein and protein-protein interactions, etc. Application of voltammetric methods, such as fast scan CV and normal pulse voltammetry showed smaller sensitivity to tiny changes in protein structures. Recently CHER was found also in some polysaccharides such as chitosan and in NH2 group-containing glycans. Very recent development in electrochemical analysis of DNA and RNA was briefly summarized. (C) 2017 Elsevier Ltd. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Materials Today

ISSN

2352-9407

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

2017

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

17

Strana od-do

434-450

Kód UT WoS článku

000417805400047

EID výsledku v databázi Scopus

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