Catalytic Deuterium Evolution and H/D Exchange in DNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00504522" target="_blank" >RIV/68081707:_____/19:00504522 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/celc.201801214" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/celc.201801214</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/celc.201801214" target="_blank" >10.1002/celc.201801214</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Catalytic Deuterium Evolution and H/D Exchange in DNA

Popis výsledku v původním jazyce

Recently, it has been shown that DNA catalyzes the hydrogen evolution reaction (HER) at mercury-containing electrodes, which provide voltammetric and chronopotentiometric stripping (CPS) peaks H-DNA, allowing label-free DNA determination at very low concentrations. Here, we compared DNA catalytic HER in H2O with catalytic deuterium evolution reaction (DER) in D2O. We show that in D2O single-stranded (ss) and double-stranded (ds) DNAs produced CPS peaks H-DNA(D) due to DER. Compared to peaks H-DNA obtained in H2O, peaks H-DNA(D) were smaller and appeared at more negative potentials. Under conditions where dsDNA did not produce any peak H-DNA(D) in D2O, this DNA produced in H2O peak H-DNA.These results suggested a lack of accessible bases in the D2O surface-attached dsDNA. This might be attributed to the greater stability of deuterated dsDNA, better resisting the electric field effects at the negatively charged interface. The process of protonation of deuterated ssDNA was very fast in contrast to that of dsDNA, which was much slower and could be followed by CPS in a time range of tens of minutes.

Název v anglickém jazyce

Catalytic Deuterium Evolution and H/D Exchange in DNA

Popis výsledku anglicky

Recently, it has been shown that DNA catalyzes the hydrogen evolution reaction (HER) at mercury-containing electrodes, which provide voltammetric and chronopotentiometric stripping (CPS) peaks H-DNA, allowing label-free DNA determination at very low concentrations. Here, we compared DNA catalytic HER in H2O with catalytic deuterium evolution reaction (DER) in D2O. We show that in D2O single-stranded (ss) and double-stranded (ds) DNAs produced CPS peaks H-DNA(D) due to DER. Compared to peaks H-DNA obtained in H2O, peaks H-DNA(D) were smaller and appeared at more negative potentials. Under conditions where dsDNA did not produce any peak H-DNA(D) in D2O, this DNA produced in H2O peak H-DNA.These results suggested a lack of accessible bases in the D2O surface-attached dsDNA. This might be attributed to the greater stability of deuterated dsDNA, better resisting the electric field effects at the negatively charged interface. The process of protonation of deuterated ssDNA was very fast in contrast to that of dsDNA, which was much slower and could be followed by CPS in a time range of tens of minutes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

<a href="/cs/project/GA17-08971S" target="_blank" >GA17-08971S: Chemická modifikace a elektrochemická analýza nukleových kyselin jako nový nástroj v molekulární onkologii</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ChemElectroChem

ISSN

2196-0216

e-ISSN

—

Svazek periodika

6

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

1032-1039

Kód UT WoS článku

000461580800010

EID výsledku v databázi Scopus

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