Label-free electrochemical analysis of purine nucleotides and nucleobases at disposable carbon electrodes in microliter volumes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00518362" target="_blank" >RIV/68081707:_____/19:00518362 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S157266571930520X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S157266571930520X?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jelechem.2019.113252" target="_blank" >10.1016/j.jelechem.2019.113252</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Label-free electrochemical analysis of purine nucleotides and nucleobases at disposable carbon electrodes in microliter volumes

Popis výsledku v původním jazyce

We analyzed purine nucleotides in oligodeoxynucleotides (ODN) and nucleobase residues acid-hydrolyzed ODN at nanomolar concentrations in few tens of microliters of solution at commercially available unmodified graphite pencil electrodes, screen printed graphite electrodes (SPGEs) and SPGEs modified by graphene, single- and multi-walled carbon nanotubes. Our results suggest that specific properties of different graphite-based electrodes have significant impact on ODN analysis. Nanostructure-modified electrodes produced higher signals due to purine nucleobase oxidation but also higher background currents. The unmodified disposable carbon electrodes allowed analysis of both free nucleobases and ODNs. Our results show that unmodified SPGEs represent a good compromise for signal/noise ratio. Electrooxidation of purines at these electrodes was only little affected by the presence of pyrimidines in contrast to the pencil graphite electrode.

Název v anglickém jazyce

Label-free electrochemical analysis of purine nucleotides and nucleobases at disposable carbon electrodes in microliter volumes

Popis výsledku anglicky

We analyzed purine nucleotides in oligodeoxynucleotides (ODN) and nucleobase residues acid-hydrolyzed ODN at nanomolar concentrations in few tens of microliters of solution at commercially available unmodified graphite pencil electrodes, screen printed graphite electrodes (SPGEs) and SPGEs modified by graphene, single- and multi-walled carbon nanotubes. Our results suggest that specific properties of different graphite-based electrodes have significant impact on ODN analysis. Nanostructure-modified electrodes produced higher signals due to purine nucleobase oxidation but also higher background currents. The unmodified disposable carbon electrodes allowed analysis of both free nucleobases and ODNs. Our results show that unmodified SPGEs represent a good compromise for signal/noise ratio. Electrooxidation of purines at these electrodes was only little affected by the presence of pyrimidines in contrast to the pencil graphite electrode.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Electroanalytical Chemistry

ISSN

1572-6657

e-ISSN

—

Svazek periodika

847

Číslo periodika v rámci svazku

AUG 15 2019

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

7

Strana od-do

113252

Kód UT WoS článku

000482532200065

EID výsledku v databázi Scopus

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