Electrodeposition of Silver Amalgam on Thin Gold Film Electrodes for Voltammetric Detection of 4-Nitrophenol and DNA Labeled with Osmium Tetroxide-Bipyridine Complex

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00518368" target="_blank" >RIV/68081707:_____/19:00518368 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/19:PU134306

Výsledek na webu

<a href="http://dspace.lib.vutbr.cz/xmlui/bitstream/11012/184643/1/2019%20Electroanalsis%20Havranova%20Preprint.pdf" target="_blank" >http://dspace.lib.vutbr.cz/xmlui/bitstream/11012/184643/1/2019%20Electroanalsis%20Havranova%20Preprint.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elan.201900306" target="_blank" >10.1002/elan.201900306</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrodeposition of Silver Amalgam on Thin Gold Film Electrodes for Voltammetric Detection of 4-Nitrophenol and DNA Labeled with Osmium Tetroxide-Bipyridine Complex

Popis výsledku v původním jazyce

Alternative electrode materials suitable to prepare novel working electrode applicable in detecting biopolymers such as nucleic acids, proteins or glycoproteins, represent a significant contribution to bio-electroanalysis. Herein, electrodes made of vapor-deposited thin gold films (vAuE) were used as an alternative substrate for the electrodeposition of silver amalgam particles (AgAPs), next to indium tin oxide and pyrolytic graphite, which are already used. The conditions and parameters of double pulse chronoamperometry were optimized for the most-sensitive voltammetric detection of 4-nitrophenol (4-NP). The resulting electrodes were characterized by scanning electron microscope with energy dispersive X-ray spectroscopy. While 4-NP could not be detected by bare nonactivated vAuEs at all, their electrochemical activation offered a limit of detection (LoD) of 25 and 5 mu mol.l(-1) by means of CV and DPV, respectively. AgAP electrodeposited on vAuE, offered 2.5-times lower LoDs 10 mu mol.l(-1) by CV and comparable LoD 5 mu mol.l(-1) by DPV. Advantageously, AgAPs could be repeatedly deposited on and anodically dissolved from the vAuE with a relative standard deviation 13 % of the ten-times repeated DPV signal of 4-NP (100 mu mol.l(-1)). In comparison to vAuE, the vAuE-AgAP offered about 400 mV broader potential window, which allowed detection of single strand DNA fragment labeled by osmium tetroxide-bipyridine complex down to 2 ng.mu l(-1) by means of DPV.

Název v anglickém jazyce

Electrodeposition of Silver Amalgam on Thin Gold Film Electrodes for Voltammetric Detection of 4-Nitrophenol and DNA Labeled with Osmium Tetroxide-Bipyridine Complex

Popis výsledku anglicky

Alternative electrode materials suitable to prepare novel working electrode applicable in detecting biopolymers such as nucleic acids, proteins or glycoproteins, represent a significant contribution to bio-electroanalysis. Herein, electrodes made of vapor-deposited thin gold films (vAuE) were used as an alternative substrate for the electrodeposition of silver amalgam particles (AgAPs), next to indium tin oxide and pyrolytic graphite, which are already used. The conditions and parameters of double pulse chronoamperometry were optimized for the most-sensitive voltammetric detection of 4-nitrophenol (4-NP). The resulting electrodes were characterized by scanning electron microscope with energy dispersive X-ray spectroscopy. While 4-NP could not be detected by bare nonactivated vAuEs at all, their electrochemical activation offered a limit of detection (LoD) of 25 and 5 mu mol.l(-1) by means of CV and DPV, respectively. AgAP electrodeposited on vAuE, offered 2.5-times lower LoDs 10 mu mol.l(-1) by CV and comparable LoD 5 mu mol.l(-1) by DPV. Advantageously, AgAPs could be repeatedly deposited on and anodically dissolved from the vAuE with a relative standard deviation 13 % of the ten-times repeated DPV signal of 4-NP (100 mu mol.l(-1)). In comparison to vAuE, the vAuE-AgAP offered about 400 mV broader potential window, which allowed detection of single strand DNA fragment labeled by osmium tetroxide-bipyridine complex down to 2 ng.mu l(-1) by means of DPV.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electroanalysis

ISSN

1040-0397

e-ISSN

—

Svazek periodika

31

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

1952-1960

Kód UT WoS článku

000474183900001

EID výsledku v databázi Scopus

—