Simultaneous voltammetric determination of free tryptophan, uric acid, xanthine and hypoxanthine in plasma and urine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F20%3A00521718" target="_blank" >RIV/68081707:_____/20:00521718 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/20:PU140080

Výsledek na webu

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DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.electacta.2019.135132" target="_blank" >10.1016/j.electacta.2019.135132</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Simultaneous voltammetric determination of free tryptophan, uric acid, xanthine and hypoxanthine in plasma and urine

Popis výsledku v původním jazyce

We have designed an improved electrochemical analysis of clinical samples in bodily fluids. The method allows reliable identification of several coexisting substances (tryptophan, uric acid, xanthine and hypoxanthine) present on different physiological levels. We prepared in situ electrogenerated graphite oxides (GrO) directly on the surface of a graphite pencil electrode. GrO presence enhances electrooxidation signals of small aromatic molecules and can shift their oxidation potentials. The oxidation potentials of tryptophan and tyrosine in their free and protein-bound forms, and free xanthine are very close to each other on common carbon-based materials. We found that free tryptophan's oxidation signal is significantly more enhanced (about 11-times) than the free tyrosine signal (only negligibly in the given conditions). Distinguishing xanthine's oxidation signal from that of the free tryptophan is allowed by their approximately 60 mV separation, sufficient for parallel determination by signal deconvolution. Additionally, we show that proteins containing tyrosine and/or tryptophan behave as electro-inactive and do not interfere with free amino acid determination on the GrO-modified graphite electrodes, while they strongly influence all species' detection on other carbon-based materials. Determining all individual compounds was possible at corresponding physiological values after sole dilution of human plasma (urine) and direct measurement with no pre-separation steps. (C) 2019 Elsevier Ltd. All rights reserved.

Název v anglickém jazyce

Simultaneous voltammetric determination of free tryptophan, uric acid, xanthine and hypoxanthine in plasma and urine

Popis výsledku anglicky

We have designed an improved electrochemical analysis of clinical samples in bodily fluids. The method allows reliable identification of several coexisting substances (tryptophan, uric acid, xanthine and hypoxanthine) present on different physiological levels. We prepared in situ electrogenerated graphite oxides (GrO) directly on the surface of a graphite pencil electrode. GrO presence enhances electrooxidation signals of small aromatic molecules and can shift their oxidation potentials. The oxidation potentials of tryptophan and tyrosine in their free and protein-bound forms, and free xanthine are very close to each other on common carbon-based materials. We found that free tryptophan's oxidation signal is significantly more enhanced (about 11-times) than the free tyrosine signal (only negligibly in the given conditions). Distinguishing xanthine's oxidation signal from that of the free tryptophan is allowed by their approximately 60 mV separation, sufficient for parallel determination by signal deconvolution. Additionally, we show that proteins containing tyrosine and/or tryptophan behave as electro-inactive and do not interfere with free amino acid determination on the GrO-modified graphite electrodes, while they strongly influence all species' detection on other carbon-based materials. Determining all individual compounds was possible at corresponding physiological values after sole dilution of human plasma (urine) and direct measurement with no pre-separation steps. (C) 2019 Elsevier Ltd. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

<a href="/cs/project/GA18-18154S" target="_blank" >GA18-18154S: Nové nástroje elektrochemické analýzy proteinových interakcí s nukleovými kyselinami a proteiny nevyžadující značení</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrochimica acta

ISSN

0013-4686

e-ISSN

—

Svazek periodika

329

Číslo periodika v rámci svazku

JAN 2020

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

135132

Kód UT WoS článku

000498391900034

EID výsledku v databázi Scopus

2-s2.0-85074152340