Catalytic and redox activity of nucleic acids at mercury electrodes: Roles of nucleobase residues

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F20%3A00524254" target="_blank" >RIV/68081707:_____/20:00524254 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S157266571931080X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S157266571931080X?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jelechem.2019.113812" target="_blank" >10.1016/j.jelechem.2019.113812</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Catalytic and redox activity of nucleic acids at mercury electrodes: Roles of nucleobase residues

Popis výsledku v původním jazyce

Using a series of specifically designed oligonucleotides we have identified adenine and cytosine nucleobases as residues involved in catalytic hydrogen evolution reaction (CHER) of nucleic acids at the hanging mercury drop electrode (HMDE). Due to the CHER, nucleic acids yield catalytic peak H-NA allowing their label-free and reagent-less analysis at low concentrations. Additionally, our results suggest that presence of the electroactive bases (adenine and cytosine) facilitates guanine reduction which is for the first time linked to a signal measurable at the HMDE the peak P. We assume that the peak P is connected with reduction of guanine to 7,8-dihydroguanine, of which reoxidation to guanine is detected at the electrode by the earlier described anodic peak G. Processes connected with the above mentioned signals were studied using cyclic voltammetry by inspection of dependences on experimental parameters such as negative vertex potential and pH. (C) 2019 Elsevier B.V. All rights reserved.

Název v anglickém jazyce

Catalytic and redox activity of nucleic acids at mercury electrodes: Roles of nucleobase residues

Popis výsledku anglicky

Using a series of specifically designed oligonucleotides we have identified adenine and cytosine nucleobases as residues involved in catalytic hydrogen evolution reaction (CHER) of nucleic acids at the hanging mercury drop electrode (HMDE). Due to the CHER, nucleic acids yield catalytic peak H-NA allowing their label-free and reagent-less analysis at low concentrations. Additionally, our results suggest that presence of the electroactive bases (adenine and cytosine) facilitates guanine reduction which is for the first time linked to a signal measurable at the HMDE the peak P. We assume that the peak P is connected with reduction of guanine to 7,8-dihydroguanine, of which reoxidation to guanine is detected at the electrode by the earlier described anodic peak G. Processes connected with the above mentioned signals were studied using cyclic voltammetry by inspection of dependences on experimental parameters such as negative vertex potential and pH. (C) 2019 Elsevier B.V. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Electroanalytical Chemistry

ISSN

1572-6657

e-ISSN

—

Svazek periodika

858

Číslo periodika v rámci svazku

FEB 1 2020

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

6

Strana od-do

113812

Kód UT WoS článku

000515205600043

EID výsledku v databázi Scopus

2-s2.0-85077739525