The fate of 35S rRNA genes in the allotetraploid grassBrachypodium hybridum
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F20%3A00539338" target="_blank" >RIV/68081707:_____/20:00539338 - isvavai.cz</a>
Výsledek na webu
<a href="https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.14869" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.14869</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/tpj.14869" target="_blank" >10.1111/tpj.14869</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The fate of 35S rRNA genes in the allotetraploid grassBrachypodium hybridum
Popis výsledku v původním jazyce
Nucleolar dominance (ND) consists of the reversible silencing of 35S/45S rDNA loci inherited from one of the ancestors of an allopolyploid. The molecular mechanisms by which one ancestral rDNA set is selected for silencing remain unclear. We applied a combination of molecular (Southern blot hybridization and reverse-transcription cleaved amplified polymorphic sequence analysis), genomic (analysis of variants) and cytogenetic (fluorescencein situhybridization) approaches to study the structure, expression and epigenetic landscape of 35S rDNA in an allotetraploid grass that exhibits ND,Brachypodium hybridum(genome composition DDSS), and its putative progenitors,Brachypodium distachyon(DD) andBrachypodium stacei(SS). In progenitor genomes,B. staceishowed a higher intragenomic heterogeneity of rDNA compared withB. distachyon. In all studied accessions ofB. hybridum, there was a reduction in the copy number of S homoeologues, which was accompanied by their inactive transcriptional status. The involvement of DNA methylation in CG and CHG contexts in the silencing of the S-genome rDNA loci was revealed. In theB. hybridumallotetraploid, ND is stabilized towards the D-genome units, irrespective of the polyphyletic origin of the species, and does not seem to be influenced by homoeologous 35S rDNA ratios and developmental stage.
Název v anglickém jazyce
The fate of 35S rRNA genes in the allotetraploid grassBrachypodium hybridum
Popis výsledku anglicky
Nucleolar dominance (ND) consists of the reversible silencing of 35S/45S rDNA loci inherited from one of the ancestors of an allopolyploid. The molecular mechanisms by which one ancestral rDNA set is selected for silencing remain unclear. We applied a combination of molecular (Southern blot hybridization and reverse-transcription cleaved amplified polymorphic sequence analysis), genomic (analysis of variants) and cytogenetic (fluorescencein situhybridization) approaches to study the structure, expression and epigenetic landscape of 35S rDNA in an allotetraploid grass that exhibits ND,Brachypodium hybridum(genome composition DDSS), and its putative progenitors,Brachypodium distachyon(DD) andBrachypodium stacei(SS). In progenitor genomes,B. staceishowed a higher intragenomic heterogeneity of rDNA compared withB. distachyon. In all studied accessions ofB. hybridum, there was a reduction in the copy number of S homoeologues, which was accompanied by their inactive transcriptional status. The involvement of DNA methylation in CG and CHG contexts in the silencing of the S-genome rDNA loci was revealed. In theB. hybridumallotetraploid, ND is stabilized towards the D-genome units, irrespective of the polyphyletic origin of the species, and does not seem to be influenced by homoeologous 35S rDNA ratios and developmental stage.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10611 - Plant sciences, botany
Návaznosti výsledku
Projekt
<a href="/cs/project/GC20-14133J" target="_blank" >GC20-14133J: Sex ano či ne - Vliv asymetrické meiosy na evoluci genů u polyploidních růží</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Plant Journal
ISSN
0960-7412
e-ISSN
—
Svazek periodika
103
Číslo periodika v rámci svazku
5
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
16
Strana od-do
1810-1825
Kód UT WoS článku
000545082000001
EID výsledku v databázi Scopus
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