Protease associated domain of RNF43 is not necessary for the suppression of Wnt/beta-catenin signaling in human cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F20%3A00540114" target="_blank" >RIV/68081707:_____/20:00540114 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/20:00114371

Výsledek na webu

<a href="https://biosignaling.biomedcentral.com/articles/10.1186/s12964-020-00559-0" target="_blank" >https://biosignaling.biomedcentral.com/articles/10.1186/s12964-020-00559-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12964-020-00559-0" target="_blank" >10.1186/s12964-020-00559-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Protease associated domain of RNF43 is not necessary for the suppression of Wnt/beta-catenin signaling in human cells

Popis výsledku v původním jazyce

BackgroundRNF43 and its homolog ZNRF3 are transmembrane E3 ubiquitin ligases frequently mutated in many human cancer types. Their main role relays on the inhibition of canonical Wnt signaling by the negative regulation of frizzled receptors and LRP5/6 co-receptors levels at the plasma membrane. Intracellular RING domains of RNF43/ZNRF3 mediate the key enzymatic activity of these proteins, but the function of the extracellular Protease Associated (PA) fold in the inhibition of Wnt/beta-catenin pathway is controversial up-to date, apart from the interaction with secreted antagonists R-spondin family proteins shown by the crystallographic studies.MethodsIn our research we utilised cell-based approaches to study the role of RNF43 lacking PA domain in the canonical Wnt signalling pathway transduction. We developed controlled overexpression (TetON) and CRISPR/Cas9 mediated knock-out models in human cells.ResultsRNF43 Delta PA mutant activity impedes canonical Wnt pathway, as manifested by the reduced phosphorylation of LRP6, DVL2 and DVL3 and by the decreased beta-catenin-dependent gene expression. Finally, rescue experiments in the CRISPR/Cas9 derived RNF43/ZNRF3 double knock-out cell lines showed that RNF Delta PA overexpression is enough to inhibit activation of LRP6 and beta-catenin activity as shown by the Western blot and Top flash dual luciferase assays. Moreover, RNF43 variant without PA domain was not sensitive to the R-spondin1 treatment.ConclusionTaken together, our results help to understand better the mode of RNF43 tumor suppressor action and solve some discrepancies present in the field.

Název v anglickém jazyce

Protease associated domain of RNF43 is not necessary for the suppression of Wnt/beta-catenin signaling in human cells

Popis výsledku anglicky

BackgroundRNF43 and its homolog ZNRF3 are transmembrane E3 ubiquitin ligases frequently mutated in many human cancer types. Their main role relays on the inhibition of canonical Wnt signaling by the negative regulation of frizzled receptors and LRP5/6 co-receptors levels at the plasma membrane. Intracellular RING domains of RNF43/ZNRF3 mediate the key enzymatic activity of these proteins, but the function of the extracellular Protease Associated (PA) fold in the inhibition of Wnt/beta-catenin pathway is controversial up-to date, apart from the interaction with secreted antagonists R-spondin family proteins shown by the crystallographic studies.MethodsIn our research we utilised cell-based approaches to study the role of RNF43 lacking PA domain in the canonical Wnt signalling pathway transduction. We developed controlled overexpression (TetON) and CRISPR/Cas9 mediated knock-out models in human cells.ResultsRNF43 Delta PA mutant activity impedes canonical Wnt pathway, as manifested by the reduced phosphorylation of LRP6, DVL2 and DVL3 and by the decreased beta-catenin-dependent gene expression. Finally, rescue experiments in the CRISPR/Cas9 derived RNF43/ZNRF3 double knock-out cell lines showed that RNF Delta PA overexpression is enough to inhibit activation of LRP6 and beta-catenin activity as shown by the Western blot and Top flash dual luciferase assays. Moreover, RNF43 variant without PA domain was not sensitive to the R-spondin1 treatment.ConclusionTaken together, our results help to understand better the mode of RNF43 tumor suppressor action and solve some discrepancies present in the field.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/GX19-28347X" target="_blank" >GX19-28347X: Molekulární a funkční analýza biologie kasein kinázy 1</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cell communication and signaling : CCS

ISSN

1478-811X

e-ISSN

—

Svazek periodika

18

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

91

Kód UT WoS článku

000542292400001

EID výsledku v databázi Scopus

2-s2.0-85086424863