Chronopotentiometric Analysis of Single Histones and Histone Octamer at Charged Surfaces

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F21%3A00546664" target="_blank" >RIV/68081707:_____/21:00546664 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/21:00122240

Výsledek na webu

<a href="https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/celc.202100817" target="_blank" >https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/celc.202100817</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/celc.202100817" target="_blank" >10.1002/celc.202100817</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chronopotentiometric Analysis of Single Histones and Histone Octamer at Charged Surfaces

Popis výsledku v původním jazyce

Histones play an essential role in the nucleosome structure and organization. The key role of highly positively charged histones is to stabilize negatively charged DNA inside the chromatin. Histones are basic proteins containing a large amount of electroactive residues, namely, arginine and lysine. Additionally, histone H3, on the contrary to other histones, contains cysteine residues. To understand more about the behavior of histones at charged surfaces and the influence of the electroactive amino acid residues content on the catalytic hydrogen evolution reaction, we studied histones using label-free approaches, namely, chronopotentiometric stripping analysis (CPS) and impedance C-d-t curves. While CPS is sensitive to the structure and stability of proteins and their complexes, C-d-t curves reflect changes of the electrical double layer at the charged surface. Application of both label-free methods appears useful for analysis of complex systems. Using a combination of these methods, we could discriminate between individual single core histones, their mixture, and their complex (octamer).

Název v anglickém jazyce

Chronopotentiometric Analysis of Single Histones and Histone Octamer at Charged Surfaces

Popis výsledku anglicky

Histones play an essential role in the nucleosome structure and organization. The key role of highly positively charged histones is to stabilize negatively charged DNA inside the chromatin. Histones are basic proteins containing a large amount of electroactive residues, namely, arginine and lysine. Additionally, histone H3, on the contrary to other histones, contains cysteine residues. To understand more about the behavior of histones at charged surfaces and the influence of the electroactive amino acid residues content on the catalytic hydrogen evolution reaction, we studied histones using label-free approaches, namely, chronopotentiometric stripping analysis (CPS) and impedance C-d-t curves. While CPS is sensitive to the structure and stability of proteins and their complexes, C-d-t curves reflect changes of the electrical double layer at the charged surface. Application of both label-free methods appears useful for analysis of complex systems. Using a combination of these methods, we could discriminate between individual single core histones, their mixture, and their complex (octamer).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ChemElectroChem

ISSN

2196-0216

e-ISSN

2196-0216

Svazek periodika

8

Číslo periodika v rámci svazku

17

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

3360-3365

Kód UT WoS článku

000696501800022

EID výsledku v databázi Scopus

2-s2.0-85115003541