Analyses of viral genomes for G-quadruplex forming sequences reveal their correlation with the type of infection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F21%3A00554894" target="_blank" >RIV/68081707:_____/21:00554894 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26210/21:PU140962 RIV/00216224:14310/21:00121411 RIV/62156489:43110/21:43919672 RIV/61988987:17310/21:A2202A6K

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0300908421000961?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0300908421000961?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biochi.2021.03.017" target="_blank" >10.1016/j.biochi.2021.03.017</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Analyses of viral genomes for G-quadruplex forming sequences reveal their correlation with the type of infection

Popis výsledku v původním jazyce

G-quadruplexes contribute to the regulation of key molecular processes. Their utilization for antiviral therapy is an emerging field of contemporary research. Here we present comprehensive analyses of the presence and localization of putative G-quadruplex forming sequences (PQS) in all viral genomes currently available in the NCBI database (including subviral agents). The G4Hunter algorithm was applied to a pool of 11,000 accessible viral genomes representing 350 Mbp in total. PQS frequencies differ across evolutionary groups of viruses, and are enriched in repeats, replication origins, 50UTRs and 30UTRs. Importantly, PQS presence and localization is connected to viral lifecycles and corresponds to the type of viral infection rather than to nucleic acid type, while viruses routinely causing persistent infections in Metazoa hosts are enriched for PQS, viruses causing acute infections are significantly depleted for PQS. The unique localization of PQS identifies the importance of G-quadruplex-based regulation of viral replication and life cycle, providing a tool for potential therapeutic targeting. (C) 2021 The Authors. Published by Elsevier B.V.

Název v anglickém jazyce

Analyses of viral genomes for G-quadruplex forming sequences reveal their correlation with the type of infection

Popis výsledku anglicky

G-quadruplexes contribute to the regulation of key molecular processes. Their utilization for antiviral therapy is an emerging field of contemporary research. Here we present comprehensive analyses of the presence and localization of putative G-quadruplex forming sequences (PQS) in all viral genomes currently available in the NCBI database (including subviral agents). The G4Hunter algorithm was applied to a pool of 11,000 accessible viral genomes representing 350 Mbp in total. PQS frequencies differ across evolutionary groups of viruses, and are enriched in repeats, replication origins, 50UTRs and 30UTRs. Importantly, PQS presence and localization is connected to viral lifecycles and corresponds to the type of viral infection rather than to nucleic acid type, while viruses routinely causing persistent infections in Metazoa hosts are enriched for PQS, viruses causing acute infections are significantly depleted for PQS. The unique localization of PQS identifies the importance of G-quadruplex-based regulation of viral replication and life cycle, providing a tool for potential therapeutic targeting. (C) 2021 The Authors. Published by Elsevier B.V.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/EF15_003%2F0000477" target="_blank" >EF15_003/0000477: Strukturní gymnastika nukleových kyselin: od molekulárních principů přes biologické funkce k terapeutickým cílům.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimie

ISSN

0300-9084

e-ISSN

1638-6183

Svazek periodika

186

Číslo periodika v rámci svazku

JUL 2021

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

15

Strana od-do

13-27

Kód UT WoS článku

000662667800002

EID výsledku v databázi Scopus

2-s2.0-85104058471