Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F22%3A00561344" target="_blank" >RIV/68081707:_____/22:00561344 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00159816:_____/22:00077652 RIV/00209805:_____/22:00079018 RIV/00216224:14110/22:00126034

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s13770-022-00458-0" target="_blank" >https://link.springer.com/article/10.1007/s13770-022-00458-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13770-022-00458-0" target="_blank" >10.1007/s13770-022-00458-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells

Popis výsledku v původním jazyce

Background The progenitors to lung airway epithelium that are capable of long-term propagation may represent an attractive source of cells for cell-based therapies, disease modeling, toxicity testing, and others. Principally, there are two main options for obtaining lung epithelial progenitors: (i) direct isolation of endogenous progenitors from human lungs and (ii) in vitro differentiation from some other cell type. The prime candidates for the second approach are pluripotent stem cells, which may provide autologous and/or allogeneic cell resource in clinically relevant quality and quantity. Methods By exploiting the differentiation potential of human embryonic stem cells (hESC), here we derived expandable lung epithelium (ELEP) and established culture conditions for their long-term propagation (more than 6 months) in a monolayer culture without a need of 3D culture conditions and/or cell sorting steps, which minimizes potential variability of the outcome. Results These hESC-derived ELEP express NK2 Homeobox 1 (NKX2.1), a marker of early lung epithelial lineage, display properties of cells in early stages of surfactant production and are able to differentiate to cells exhibitting molecular and morphological characteristics of both respiratory epithelium of airway and alveolar regions. Conclusion Expandable lung epithelium thus offer a stable, convenient, easily scalable and high-yielding cell source for applications in biomedicine.

Název v anglickém jazyce

Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells

Popis výsledku anglicky

Background The progenitors to lung airway epithelium that are capable of long-term propagation may represent an attractive source of cells for cell-based therapies, disease modeling, toxicity testing, and others. Principally, there are two main options for obtaining lung epithelial progenitors: (i) direct isolation of endogenous progenitors from human lungs and (ii) in vitro differentiation from some other cell type. The prime candidates for the second approach are pluripotent stem cells, which may provide autologous and/or allogeneic cell resource in clinically relevant quality and quantity. Methods By exploiting the differentiation potential of human embryonic stem cells (hESC), here we derived expandable lung epithelium (ELEP) and established culture conditions for their long-term propagation (more than 6 months) in a monolayer culture without a need of 3D culture conditions and/or cell sorting steps, which minimizes potential variability of the outcome. Results These hESC-derived ELEP express NK2 Homeobox 1 (NKX2.1), a marker of early lung epithelial lineage, display properties of cells in early stages of surfactant production and are able to differentiate to cells exhibitting molecular and morphological characteristics of both respiratory epithelium of airway and alveolar regions. Conclusion Expandable lung epithelium thus offer a stable, convenient, easily scalable and high-yielding cell source for applications in biomedicine.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Jo'jig Gonghag gwa Jaesaeng Uihag

ISSN

1738-2696

e-ISSN

2212-5469

Svazek periodika

19

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

KR - Korejská republika

Počet stran výsledku

18

Strana od-do

1033-1050

Kód UT WoS článku

000807342200001

EID výsledku v databázi Scopus

2-s2.0-85131534857