Compromised function of ARM, the interactor of Arabidopsis telomerase, suggests its role in stress responses
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F22%3A00562612" target="_blank" >RIV/68081707:_____/22:00562612 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216224:14310/22:00128593
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/pii/S0168945222002783?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0168945222002783?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.plantsci.2022.111453" target="_blank" >10.1016/j.plantsci.2022.111453</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Compromised function of ARM, the interactor of Arabidopsis telomerase, suggests its role in stress responses
Popis výsledku v původním jazyce
ARM was identified previously as an interaction partner of the telomerase protein subunit (TERT) in Arabidopsis thaliana. To investigate the interconnection between ARM and telomerase and to identify ARM cellular functions, we analyzed a set of arm mutant lines and arm/tert double mutants. Telomere length was not affected in arm single mutant plants, in contrast to double mutants. In the second generation of homozygous arm-1/tert double mutants following the heterozygous state during the double mutant construction, telomeres shortened dramat-ically, even below levels in tert plants displaying severe morphological defects. Intriguingly, homozygous arm-1/ tert double mutants with short telomeres grew without obvious phenotypic changes for next two generations. Then, in agreement with the onset of phenotypic changes in tert, morphological defects were timed to the 5th arm-1/tert homozygous generation. RNAseq analyses of arm-1/tert and respective single mutants displayed markedly overlapping sets of differentially expressed genes in arm-1/tert double mutant and arm-1 single mutant lines, indicating a dominant effect of the ARM mutation. RNAseq data further implied ARM involvement in circadian rhythms, responses to drugs and to biotic and abiotic stimuli. In agreement with it, we observed sensitivity of arm-1 single mutant to the heat stress during germination. Altogether, our results suggest ARM involvement in crucial cellular processes without evidencing its role in the telomerase canonical function.
Název v anglickém jazyce
Compromised function of ARM, the interactor of Arabidopsis telomerase, suggests its role in stress responses
Popis výsledku anglicky
ARM was identified previously as an interaction partner of the telomerase protein subunit (TERT) in Arabidopsis thaliana. To investigate the interconnection between ARM and telomerase and to identify ARM cellular functions, we analyzed a set of arm mutant lines and arm/tert double mutants. Telomere length was not affected in arm single mutant plants, in contrast to double mutants. In the second generation of homozygous arm-1/tert double mutants following the heterozygous state during the double mutant construction, telomeres shortened dramat-ically, even below levels in tert plants displaying severe morphological defects. Intriguingly, homozygous arm-1/ tert double mutants with short telomeres grew without obvious phenotypic changes for next two generations. Then, in agreement with the onset of phenotypic changes in tert, morphological defects were timed to the 5th arm-1/tert homozygous generation. RNAseq analyses of arm-1/tert and respective single mutants displayed markedly overlapping sets of differentially expressed genes in arm-1/tert double mutant and arm-1 single mutant lines, indicating a dominant effect of the ARM mutation. RNAseq data further implied ARM involvement in circadian rhythms, responses to drugs and to biotic and abiotic stimuli. In agreement with it, we observed sensitivity of arm-1 single mutant to the heat stress during germination. Altogether, our results suggest ARM involvement in crucial cellular processes without evidencing its role in the telomerase canonical function.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Plant Science
ISSN
0168-9452
e-ISSN
1873-2259
Svazek periodika
325
Číslo periodika v rámci svazku
DEC 2022
Stát vydavatele periodika
IE - Irsko
Počet stran výsledku
16
Strana od-do
111453
Kód UT WoS článku
000862382300002
EID výsledku v databázi Scopus
2-s2.0-85138552413