Early recruitment of PARP-dependent m(8)A RNA methylation at DNA lesions is subsequently accompanied by active DNA demethylation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F22%3A00565104" target="_blank" >RIV/68081707:_____/22:00565104 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.tandfonline.com/doi/full/10.1080/15476286.2022.2139109" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/15476286.2022.2139109</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/15476286.2022.2139109" target="_blank" >10.1080/15476286.2022.2139109</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Early recruitment of PARP-dependent m(8)A RNA methylation at DNA lesions is subsequently accompanied by active DNA demethylation

Popis výsledku v původním jazyce

RNA methylation, especially 6-methyladenosine (m(6)A)-modified RNAs, plays a specific role in DNA damage response (DDR). Here, we also observe that RNA modified at 8-methyladenosine (m(8)A) is recruited to UVA-damaged chromatin immediately after microirradiation. Interestingly, the level of m(8)A RNA at genomic lesions was reduced after inhibition of histone deacetylases and DNA methyltransferases. It appears in later phases of DNA damage response, accompanied by active DNA demethylation. Also, PARP inhibitor (PARPi), Olaparib, prevented adenosine methylation at microirradiated chromatin. PARPi abrogated not only m(6)A and m(8)A RNA positivity at genomic lesions, but also XRCC1, the factor of base excision repair (BER), did not recognize lesions in DNA. To this effect, Olaparib enhanced the genome-wide level of gamma H2AX. This histone modification interacted with m(8)A RNAs to a similar extent as m(8)A RNAs with DNA. Pronounced interaction properties we did not observe for m(6)A RNAs and DNA, however, m(6)A RNA interacted with XRCC1 with the highest efficiency, especially in microirradiated cells. Together, we show that the recruitment of m(6)A RNA and m(8)A RNA to DNA lesions is PARP dependent. We suggest that modified RNAs likely play a role in the BER mechanism accompanied by active DNA demethylation. In this process, gamma H2AX stabilizes m(6)A/m(8)A-positive RNA-DNA hybrid loops via its interaction with m(8)A RNAs. R-loops could represent basic three-stranded structures recognized by PARP-dependent non-canonical m(6)A/m(8)A-mediated DNA repair pathway.

Název v anglickém jazyce

Early recruitment of PARP-dependent m(8)A RNA methylation at DNA lesions is subsequently accompanied by active DNA demethylation

Popis výsledku anglicky

RNA methylation, especially 6-methyladenosine (m(6)A)-modified RNAs, plays a specific role in DNA damage response (DDR). Here, we also observe that RNA modified at 8-methyladenosine (m(8)A) is recruited to UVA-damaged chromatin immediately after microirradiation. Interestingly, the level of m(8)A RNA at genomic lesions was reduced after inhibition of histone deacetylases and DNA methyltransferases. It appears in later phases of DNA damage response, accompanied by active DNA demethylation. Also, PARP inhibitor (PARPi), Olaparib, prevented adenosine methylation at microirradiated chromatin. PARPi abrogated not only m(6)A and m(8)A RNA positivity at genomic lesions, but also XRCC1, the factor of base excision repair (BER), did not recognize lesions in DNA. To this effect, Olaparib enhanced the genome-wide level of gamma H2AX. This histone modification interacted with m(8)A RNAs to a similar extent as m(8)A RNAs with DNA. Pronounced interaction properties we did not observe for m(6)A RNAs and DNA, however, m(6)A RNA interacted with XRCC1 with the highest efficiency, especially in microirradiated cells. Together, we show that the recruitment of m(6)A RNA and m(8)A RNA to DNA lesions is PARP dependent. We suggest that modified RNAs likely play a role in the BER mechanism accompanied by active DNA demethylation. In this process, gamma H2AX stabilizes m(6)A/m(8)A-positive RNA-DNA hybrid loops via its interaction with m(8)A RNAs. R-loops could represent basic three-stranded structures recognized by PARP-dependent non-canonical m(6)A/m(8)A-mediated DNA repair pathway.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

RNA biology

ISSN

1547-6286

e-ISSN

1555-8584

Svazek periodika

19

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

19

Strana od-do

1153-1171

Kód UT WoS článku

000884574000001

EID výsledku v databázi Scopus

—