Structural and dynamic effects of pseudouridine modifications on noncanonical interactions in RNA
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F23%3A00574517" target="_blank" >RIV/68081707:_____/23:00574517 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216224:14740/23:00130997
Výsledek na webu
<a href="https://rnajournal.cshlp.org/content/29/6/790" target="_blank" >https://rnajournal.cshlp.org/content/29/6/790</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1261/rna.079506.122" target="_blank" >10.1261/rna.079506.122</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Structural and dynamic effects of pseudouridine modifications on noncanonical interactions in RNA
Popis výsledku v původním jazyce
Pseudouridine is the most frequently naturally occurring RNA modification, found in all classes of biologically functional RNAs. Compared to uridine, pseudouridine contains an additional hydrogen bond donor group and is therefore widely regarded as a structure stabilizing modification. However, the effects of pseudouridine modifications on the structure and dynamics of RNAs have so far only been investigated in a limited number of different structural contexts. Here, we introduced pseudouridine modifications into the U-turn motif and the adjacent U:U closing base pair of the neomycin-sensing riboswitch (NSR)-an extensively characterized model system for RNA structure, ligand binding, and dynamics. We show that the effects of replacing specific uridines with pseudouridines on RNA dynamics crucially depend on the exact location of the replacement site and can range from destabilizing to locally or even globally stabilizing. By using a combination of NMR spectroscopy, MD simulations and QM calculations, we rationalize the observed effects on a structural and dynamical level. Our results will help to better understand and predict the consequences of pseudouridine modifications on the structure and function of biologically important RNAs.
Název v anglickém jazyce
Structural and dynamic effects of pseudouridine modifications on noncanonical interactions in RNA
Popis výsledku anglicky
Pseudouridine is the most frequently naturally occurring RNA modification, found in all classes of biologically functional RNAs. Compared to uridine, pseudouridine contains an additional hydrogen bond donor group and is therefore widely regarded as a structure stabilizing modification. However, the effects of pseudouridine modifications on the structure and dynamics of RNAs have so far only been investigated in a limited number of different structural contexts. Here, we introduced pseudouridine modifications into the U-turn motif and the adjacent U:U closing base pair of the neomycin-sensing riboswitch (NSR)-an extensively characterized model system for RNA structure, ligand binding, and dynamics. We show that the effects of replacing specific uridines with pseudouridines on RNA dynamics crucially depend on the exact location of the replacement site and can range from destabilizing to locally or even globally stabilizing. By using a combination of NMR spectroscopy, MD simulations and QM calculations, we rationalize the observed effects on a structural and dynamical level. Our results will help to better understand and predict the consequences of pseudouridine modifications on the structure and function of biologically important RNAs.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
<a href="/cs/project/GA23-05639S" target="_blank" >GA23-05639S: Molekulové simulace RNA: od statických struktur k molekulárním souborům</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
RNA
ISSN
1355-8382
e-ISSN
1469-9001
Svazek periodika
29
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
18
Strana od-do
790-807
Kód UT WoS článku
000989437200007
EID výsledku v databázi Scopus
2-s2.0-85159738407