Genosensing on a 3D-printed nanocarbon electrode

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F23%3A00575169" target="_blank" >RIV/68081707:_____/23:00575169 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989100:27240/23:10253025 RIV/00216305:26620/23:PU150072

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1388248123000826?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1388248123000826?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.elecom.2023.107508" target="_blank" >10.1016/j.elecom.2023.107508</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Genosensing on a 3D-printed nanocarbon electrode

Popis výsledku v původním jazyce

In this paper we present the characterization of 3D-printed nanocarbon electrodes (3DnCes) and their application in electrochemical enzyme-linked detection of DNA hybridization. The approach takes advantage of a facile procedure based on adsorption of target DNA on the electrode surface followed by hybridization with a biotinylated probe and binding of streptavidin-alkaline phosphatase conjugate. The alkaline phosphatase converts 1-naphthyl phosphate in the background electrolyte into electrochemically oxidizable 1-naphthol, which is subsequently detected using linear sweep voltammetry. The preparation, characterization, and analytical performance of the 3DnCes are reported. The results show the applicability of such 3DnCes in detection of target DNA hybridization specifically with the complementary biotinylated probe, and indicate the potential of 3D printed electrodes for use in various bioanalytical approaches.

Název v anglickém jazyce

Genosensing on a 3D-printed nanocarbon electrode

Popis výsledku anglicky

In this paper we present the characterization of 3D-printed nanocarbon electrodes (3DnCes) and their application in electrochemical enzyme-linked detection of DNA hybridization. The approach takes advantage of a facile procedure based on adsorption of target DNA on the electrode surface followed by hybridization with a biotinylated probe and binding of streptavidin-alkaline phosphatase conjugate. The alkaline phosphatase converts 1-naphthyl phosphate in the background electrolyte into electrochemically oxidizable 1-naphthol, which is subsequently detected using linear sweep voltammetry. The preparation, characterization, and analytical performance of the 3DnCes are reported. The results show the applicability of such 3DnCes in detection of target DNA hybridization specifically with the complementary biotinylated probe, and indicate the potential of 3D printed electrodes for use in various bioanalytical approaches.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

<a href="/cs/project/GA20-03187S" target="_blank" >GA20-03187S: Studium elektrochemických a adsorpčních dějů na nanostrukturovaných sp2 a sp3 uhlíkových površích ve vztahu k jejich fyzikálně chemickým vlastnostem</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrochemistry Communications

ISSN

1388-2481

e-ISSN

1873-1902

Svazek periodika

151

Číslo periodika v rámci svazku

JUN 2023

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

107508

Kód UT WoS článku

001055116600001

EID výsledku v databázi Scopus

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