In Planta Reporter Assay Demonstrated the Inherent Potential of Turmeric as a Natural Hypo-Methylating Agent in Cultivated Tobacco (Nicotiana tabacum L.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F24%3A00618134" target="_blank" >RIV/68081707:_____/24:00618134 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1134/S1021443724608279" target="_blank" >https://link.springer.com/article/10.1134/S1021443724608279</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1134/S1021443724608279" target="_blank" >10.1134/S1021443724608279</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In Planta Reporter Assay Demonstrated the Inherent Potential of Turmeric as a Natural Hypo-Methylating Agent in Cultivated Tobacco (Nicotiana tabacum L.)

Popis výsledku v původním jazyce

The current research seeks to assess the inherent potential (if any) of turmeric (TMC) as an epigenetic modulator in plants. To monitor its specific effects on gene-activation, we used a well-established in planta reporter assay system based on cultivated tobacco (Nicotiana tabacum L.) as a model system harbouring an epigenetically-silenced (hyper-methylation in specific) neomycin phosphotransferase (nptII) transgene driven by cauliflower mosaic virus 35S promoter (P-CaMV35S). Our results showed that the exposure of tobacco plantlets to an empirically optimized concentration [i.e., 2.5% (v/v)] of aqueous TMC extract (TMCE) causes the: (1) restoration of non-silenced plant phenotype on kanamycin selection media, (2) induced steady-state nptII transcript levels and (3) DNA hypo-methylation of 5'-UTR nptII sub-region. Based on these observations, a general role of TMC as a DNA hypo-methylating agent in plants growth and development has been anticipated.

Název v anglickém jazyce

In Planta Reporter Assay Demonstrated the Inherent Potential of Turmeric as a Natural Hypo-Methylating Agent in Cultivated Tobacco (Nicotiana tabacum L.)

Popis výsledku anglicky

The current research seeks to assess the inherent potential (if any) of turmeric (TMC) as an epigenetic modulator in plants. To monitor its specific effects on gene-activation, we used a well-established in planta reporter assay system based on cultivated tobacco (Nicotiana tabacum L.) as a model system harbouring an epigenetically-silenced (hyper-methylation in specific) neomycin phosphotransferase (nptII) transgene driven by cauliflower mosaic virus 35S promoter (P-CaMV35S). Our results showed that the exposure of tobacco plantlets to an empirically optimized concentration [i.e., 2.5% (v/v)] of aqueous TMC extract (TMCE) causes the: (1) restoration of non-silenced plant phenotype on kanamycin selection media, (2) induced steady-state nptII transcript levels and (3) DNA hypo-methylation of 5'-UTR nptII sub-region. Based on these observations, a general role of TMC as a DNA hypo-methylating agent in plants growth and development has been anticipated.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/GA20-28029S" target="_blank" >GA20-28029S: Význam zdvojených promotorových sekvencí pro funkci rostlinných mezigenových mezerníků u 35S rDNA</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Russian Journal of Plant Physiology

ISSN

1021-4437

e-ISSN

1608-3407

Svazek periodika

71

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

RU - Ruská federace

Počet stran výsledku

7

Strana od-do

198

Kód UT WoS článku

001412539000030

EID výsledku v databázi Scopus

2-s2.0-85218160266