Why robust background electrolytes containing multivalent ionic species can fail in capillary zone electrophoresis.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F01%3A25010008" target="_blank" >RIV/68081715:_____/01:25010008 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Why robust background electrolytes containing multivalent ionic species can fail in capillary zone electrophoresis.

Popis výsledku v původním jazyce

In this paper it is demonstrated that system peaks are inducted by multivalent weak ionic species in background electrolytes. Their existence is derived from SystCharts and from peak shape diagrams and the theory is confirmed experimentally. If analytesare present in a sample, with mobilities approximately equal to the mobility of a system peak, they interact, resulting in a strong increase of electromigration dispersion. This leads to a strong peak broadening, peak deformation and a loss of resolution. Typical background electrolytes containing multivalent ionic species, e.g. phosphate and phthalate buffers, in literature ofte proposed to be robust electrolytes, are therefore not always universally applicable and can fail for the application of specific analytes. This paper brings a systematic study of the above phenomena and shows theoretical and experimental results for BGEs containing phosphoric acid and phthalic acid.

Název v anglickém jazyce

Why robust background electrolytes containing multivalent ionic species can fail in capillary zone electrophoresis.

Popis výsledku anglicky

In this paper it is demonstrated that system peaks are inducted by multivalent weak ionic species in background electrolytes. Their existence is derived from SystCharts and from peak shape diagrams and the theory is confirmed experimentally. If analytesare present in a sample, with mobilities approximately equal to the mobility of a system peak, they interact, resulting in a strong increase of electromigration dispersion. This leads to a strong peak broadening, peak deformation and a loss of resolution. Typical background electrolytes containing multivalent ionic species, e.g. phosphate and phthalate buffers, in literature ofte proposed to be robust electrolytes, are therefore not always universally applicable and can fail for the application of specific analytes. This paper brings a systematic study of the above phenomena and shows theoretical and experimental results for BGEs containing phosphoric acid and phthalic acid.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Svazek periodika

916

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

41-49

Kód UT WoS článku

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EID výsledku v databázi Scopus

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