Standards for capillary isoelectric focusing with laser induced fluorescence detection.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F01%3A25010051" target="_blank" >RIV/68081715:_____/01:25010051 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26310/01:PU22169

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Standards for capillary isoelectric focusing with laser induced fluorescence detection.

Popis výsledku v původním jazyce

Proteins, peptides or BGE ampholytes labeled with suitable dyes were previously suggested as pI markers. However, both proteins and background ampholytes have many possible reactive sites for reactive dyes and this might result in formation of heterogeneously labeled product with respect to pI. By contrast, the low molecular pI markers can be prepared with heterogeneity and greater stability than proteins and could potentially be good pI markers as found in case of UV detection. To improve the laser induced fluorescence detection, new low molecular fluorescent compounds excitable around 490 nm with suitable acidobasic and electrophoretic properties were prepared and focused in capillary IEF with photometric or fluorometric detection. The experimental setup of IEF and properties of new laser induced fluorescent pI markers are given.

Název v anglickém jazyce

Standards for capillary isoelectric focusing with laser induced fluorescence detection.

Popis výsledku anglicky

Proteins, peptides or BGE ampholytes labeled with suitable dyes were previously suggested as pI markers. However, both proteins and background ampholytes have many possible reactive sites for reactive dyes and this might result in formation of heterogeneously labeled product with respect to pI. By contrast, the low molecular pI markers can be prepared with heterogeneity and greater stability than proteins and could potentially be good pI markers as found in case of UV detection. To improve the laser induced fluorescence detection, new low molecular fluorescent compounds excitable around 490 nm with suitable acidobasic and electrophoretic properties were prepared and focused in capillary IEF with photometric or fluorometric detection. The experimental setup of IEF and properties of new laser induced fluorescent pI markers are given.

Druh

D - Stať ve sborníku

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/IAA4031901" target="_blank" >IAA4031901: Kapilární izotachoforéza s kombinovanými kontinuálními gradienty vodivosti a pH</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

2nd International Symposium Separations in the BioSciences. SBS 2001.

ISBN

80-7080-437-8

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

81

Název nakladatele

N/A

Místo vydání

Praha

Místo konání akce

Praha [CZ]

Datum konání akce

17. 9. 2001

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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