Dynamics of gel isoelectric focusing with ampholytic dyes monitored by camera in real-time.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F03%3A25033038" target="_blank" >RIV/68081715:_____/03:25033038 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Dynamics of gel isoelectric focusing with ampholytic dyes monitored by camera in real-time.

Popis výsledku v původním jazyce

The dynamics of gel isoelectric focusing were studied by using amphoteric low-molecular-mass colored substances (isoelectric point markers).The polyacrylamide gel in slab format was in direct contact with the electrodes. In addition to isoelectric focusing with pH gradient composed of synthetic carrier ampholytes, pH gradients created by simple buffers were applied. The progress of the elerctrofocusing process was monitored by CCD camera and video recording. The gradient profile and dynamics were approximated from the positions of isoelectric point markers. The obtained animated records enabled the observation of the entire real focusing run within fractions of a minute, which is useful both for the understanding and optimization of the focusing.

Název v anglickém jazyce

Dynamics of gel isoelectric focusing with ampholytic dyes monitored by camera in real-time.

Popis výsledku anglicky

The dynamics of gel isoelectric focusing were studied by using amphoteric low-molecular-mass colored substances (isoelectric point markers).The polyacrylamide gel in slab format was in direct contact with the electrodes. In addition to isoelectric focusing with pH gradient composed of synthetic carrier ampholytes, pH gradients created by simple buffers were applied. The progress of the elerctrofocusing process was monitored by CCD camera and video recording. The gradient profile and dynamics were approximated from the positions of isoelectric point markers. The obtained animated records enabled the observation of the entire real focusing run within fractions of a minute, which is useful both for the understanding and optimization of the focusing.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Svazek periodika

1008

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

193-203

Kód UT WoS článku

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EID výsledku v databázi Scopus

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