SDS-PAGE and gel IEF – tool for differentiation of methicillin-resistant and methicillin-sensitive strains of Staphylococcus aureus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F16%3A00449521" target="_blank" >RIV/68081715:_____/16:00449521 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00159816:_____/16:00064261 RIV/00216224:14110/16:00087691

Výsledek na webu

<a href="http://hdl.handle.net/11104/0251069" target="_blank" >http://hdl.handle.net/11104/0251069</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00284-015-0939-y" target="_blank" >10.1007/s00284-015-0939-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

SDS-PAGE and gel IEF – tool for differentiation of methicillin-resistant and methicillin-sensitive strains of Staphylococcus aureus

Popis výsledku v původním jazyce

The methicillin-resistant Staphylococcus aureus causes difficult-to-treat healthcare-associated infections in humans. For fast and effective selection of an appropriate antibiotic therapy, it is essential to have rapid and reliable methods for differentiation of methicillin-resistant Staphylococcus aureus from less dangerous methicillin-sensitive Staphylococcus aureus. There have been many methods for the identification of methicillin-resistant Staphylococcus aureus described but none has been accepted as an international standard. The most commonly used techniques such as phenotyping and genotyping have a few disadvantages, for instance, these techniques are not reproducible and stable. In addition, they are time-consuming, expensive, and they are not capable to distinguish all Staphylococcus aureus strains. In this study, the methicillin-resistant and methicillin-sensitive Staphylococcus aureus isolates obtained from patients were extracted in hot water. The released proteins were characterized by sodium dodecylsulphate polyacrylamide gel electrophoresis and gel isoelectric focusing. These two methods were able to differentiate among tested bacterial strains. The proposed methods are time saving, they are applicable in standard biochemical laboratories, and they do not require any expensive equipment.

Název v anglickém jazyce

SDS-PAGE and gel IEF – tool for differentiation of methicillin-resistant and methicillin-sensitive strains of Staphylococcus aureus

Popis výsledku anglicky

The methicillin-resistant Staphylococcus aureus causes difficult-to-treat healthcare-associated infections in humans. For fast and effective selection of an appropriate antibiotic therapy, it is essential to have rapid and reliable methods for differentiation of methicillin-resistant Staphylococcus aureus from less dangerous methicillin-sensitive Staphylococcus aureus. There have been many methods for the identification of methicillin-resistant Staphylococcus aureus described but none has been accepted as an international standard. The most commonly used techniques such as phenotyping and genotyping have a few disadvantages, for instance, these techniques are not reproducible and stable. In addition, they are time-consuming, expensive, and they are not capable to distinguish all Staphylococcus aureus strains. In this study, the methicillin-resistant and methicillin-sensitive Staphylococcus aureus isolates obtained from patients were extracted in hot water. The released proteins were characterized by sodium dodecylsulphate polyacrylamide gel electrophoresis and gel isoelectric focusing. These two methods were able to differentiate among tested bacterial strains. The proposed methods are time saving, they are applicable in standard biochemical laboratories, and they do not require any expensive equipment.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Current Microbiology

ISSN

0343-8651

e-ISSN

—

Svazek periodika

72

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

315-320

Kód UT WoS článku

000371251200011

EID výsledku v databázi Scopus

2-s2.0-84957870159