Identification of bacterial uropathogens by preparative isoelectricfocusing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F18%3A00484140" target="_blank" >RIV/68081715:_____/18:00484140 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/18:00106895 RIV/00159816:_____/18:00068884

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.chroma.2017.11.072" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2017.11.072</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2017.11.072" target="_blank" >10.1016/j.chroma.2017.11.072</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification of bacterial uropathogens by preparative isoelectricfocusing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Popis výsledku v původním jazyce

This study describes a new method for simultaneous identification of uropathogens in the case of poly-bacterial urinary tract infections. The method utilizes recently developed preparative isoelectric focusing(IEF) in cellulose-based separation medium with a subsequent analysis by matrix-assisted laser desorp-tion/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Preparative IEF was successfully usedfor both purification and separation of bacteria, Escherichia coli (pI 4.6) and Staphylococcus aureus (pI 3.4),in urine samples. The focused zones of bacteria, localized by the positions of focused colored pI markers,were easily collected from the separation media after the IEF analysis and then unambiguously identifiedby MALDI-TOF MS. The proposed method enables the identification of bacteria in urine specimens whenthe concentration of individual bacteria is ≥104cells mL−1. Another benefit is the viability of bacteriaextracted from the collected fractions after preparative IEF.

Název v anglickém jazyce

Identification of bacterial uropathogens by preparative isoelectricfocusing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Popis výsledku anglicky

This study describes a new method for simultaneous identification of uropathogens in the case of poly-bacterial urinary tract infections. The method utilizes recently developed preparative isoelectric focusing(IEF) in cellulose-based separation medium with a subsequent analysis by matrix-assisted laser desorp-tion/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Preparative IEF was successfully usedfor both purification and separation of bacteria, Escherichia coli (pI 4.6) and Staphylococcus aureus (pI 3.4),in urine samples. The focused zones of bacteria, localized by the positions of focused colored pI markers,were easily collected from the separation media after the IEF analysis and then unambiguously identifiedby MALDI-TOF MS. The proposed method enables the identification of bacteria in urine specimens whenthe concentration of individual bacteria is ≥104cells mL−1. Another benefit is the viability of bacteriaextracted from the collected fractions after preparative IEF.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1532

Číslo periodika v rámci svazku

JAN

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

232-237

Kód UT WoS článku

000423894500026

EID výsledku v databázi Scopus

2-s2.0-85036635911