Immobilization of a phosphonium ionic liquid on a silica monolith forhydrophilic interaction chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F18%3A00490130" target="_blank" >RIV/68081715:_____/18:00490130 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.chroma.2018.04.012" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2018.04.012</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2018.04.012" target="_blank" >10.1016/j.chroma.2018.04.012</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Immobilization of a phosphonium ionic liquid on a silica monolith forhydrophilic interaction chromatography

Popis výsledku v původním jazyce

A methodology for preparing phosphonium-based ionic liquid modified silica-based monolithic cap-illary columns is presented. The silica monolithic columns with dimensions of 150 × 0.1 mm weremodified by a phosphonium-based ionic liquid (trioctyl(3/4-vinylbenzyl)phosphonium chloride) via3-(trimethoxysilyl)propyl methacrylate. The prepared columns were evaluated under hydrophilic inter-action liquid chromatography separation conditions, employing a sample mixture containing purine andpyrimidine bases and nucleosides. Detection was made by UV. The high efficiency of the original silicamonolith was preserved even after modification, and it reached values in the range of 98,000–174,000theoretical plates/m. The effects of the concentration of acetonitrile in the mobile phase, the presence ofadditives in the mobile phase, such as, acetic acid or ammonium acetate, and the pH of the mobile phaseon the separation of some selected analytes were investigated. The prepared columns showed differentseparation selectivity compared to silica, phenyl and sulfobetaine stationary phases.

Název v anglickém jazyce

Immobilization of a phosphonium ionic liquid on a silica monolith forhydrophilic interaction chromatography

Popis výsledku anglicky

A methodology for preparing phosphonium-based ionic liquid modified silica-based monolithic cap-illary columns is presented. The silica monolithic columns with dimensions of 150 × 0.1 mm weremodified by a phosphonium-based ionic liquid (trioctyl(3/4-vinylbenzyl)phosphonium chloride) via3-(trimethoxysilyl)propyl methacrylate. The prepared columns were evaluated under hydrophilic inter-action liquid chromatography separation conditions, employing a sample mixture containing purine andpyrimidine bases and nucleosides. Detection was made by UV. The high efficiency of the original silicamonolith was preserved even after modification, and it reached values in the range of 98,000–174,000theoretical plates/m. The effects of the concentration of acetonitrile in the mobile phase, the presence ofadditives in the mobile phase, such as, acetic acid or ammonium acetate, and the pH of the mobile phaseon the separation of some selected analytes were investigated. The prepared columns showed differentseparation selectivity compared to silica, phenyl and sulfobetaine stationary phases.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1552

Číslo periodika v rámci svazku

JUN

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

53-59

Kód UT WoS článku

000431939400006

EID výsledku v databázi Scopus

2-s2.0-85045085429