Determination of the main phase transition temperature of phospholipids by nanoplasmonic sensing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F18%3A00494894" target="_blank" >RIV/68081715:_____/18:00494894 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1038/s41598-018-33107-5" target="_blank" >http://dx.doi.org/10.1038/s41598-018-33107-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-018-33107-5" target="_blank" >10.1038/s41598-018-33107-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of the main phase transition temperature of phospholipids by nanoplasmonic sensing

Popis výsledku v původním jazyce

Our study demonstrates that nanoplasmonic sensing (NPS) can be utilized for the determination of the phase transition temperature (T-m) of phospholipids. During the phase transition, the lipid bilayer undergoes a conformational change. Therefore, it is presumed that the T-m of phospholipids can be determined by detecting conformational changes in liposomes. The studied lipids included 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC). Liposomes in gel phase are immobilized onto silicon dioxide sensors and the sensor cell temperature is increased until passing the T-m of the lipid. The results show that, when the system temperature approaches the T-m, a drop of the NPS signal is observed. The breakpoints in the temperatures are 22.5 degrees C, 41.0 degrees C, and 55.5 degrees C for DMPC, DPPC, and DSPC, respectively. These values are very close to the theoretical T-m values, i.e., 24 degrees C, 41.4 degrees C, and 55 degrees C for DMPC, DPPC, and DSPC, respectively. Our studies prove that the NPS methodology is a simple and valuable tool for the determination of the T-m of phospholipids.

Název v anglickém jazyce

Determination of the main phase transition temperature of phospholipids by nanoplasmonic sensing

Popis výsledku anglicky

Our study demonstrates that nanoplasmonic sensing (NPS) can be utilized for the determination of the phase transition temperature (T-m) of phospholipids. During the phase transition, the lipid bilayer undergoes a conformational change. Therefore, it is presumed that the T-m of phospholipids can be determined by detecting conformational changes in liposomes. The studied lipids included 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC). Liposomes in gel phase are immobilized onto silicon dioxide sensors and the sensor cell temperature is increased until passing the T-m of the lipid. The results show that, when the system temperature approaches the T-m, a drop of the NPS signal is observed. The breakpoints in the temperatures are 22.5 degrees C, 41.0 degrees C, and 55.5 degrees C for DMPC, DPPC, and DSPC, respectively. These values are very close to the theoretical T-m values, i.e., 24 degrees C, 41.4 degrees C, and 55 degrees C for DMPC, DPPC, and DSPC, respectively. Our studies prove that the NPS methodology is a simple and valuable tool for the determination of the T-m of phospholipids.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

OCT

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

—

Kód UT WoS článku

000446325500027

EID výsledku v databázi Scopus

2-s2.0-85054446384