Down-regulation of vimentin by triorganotin isothiocyanates—nuclear retinoid X receptor agonists: A proteomic approach

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F20%3A00510284" target="_blank" >RIV/68081715:_____/20:00510284 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14160/20:00118220 RIV/62157124:16370/20:43878591

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0378427419303236?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0378427419303236?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.toxlet.2019.10.004" target="_blank" >10.1016/j.toxlet.2019.10.004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Down-regulation of vimentin by triorganotin isothiocyanates—nuclear retinoid X receptor agonists: A proteomic approach

Popis výsledku v původním jazyce

An attempt has been made to delineate the role of natural and synthetic retinoid receptor ligands on vimentin expression in the human triple-negative breast cancer cells. The effects of currently synthesized triorganotinnderivatives of the general formula R3SnX (R is butyl or phenyl, X is isothiocyanate), which are considered RXR igands, were investigated in the human MDA-MB-231 breast cancer cell line. Studies were evaluated in thenpresence and absence of all-trans retinoic acid (ATRA), a natural RAR ligand. Vimentin represents the major protein associated with epithelial-mesenchymal transition (EMT), an essential process when the primary tumourntransforms into a malignant one. mRNA and proteomic data obtained in this study, based on the PDQuest software protein evaluation and further quantification of proteins by iTRAQ analysis, suggest that vimentin wasnsignificantly reduced in the combination of RAR ligand and RXR ligand treatment. Both tested triorganotin compounds showed similarly reduced expression of vimentin, but tributyltin isothiocyanate (TBT-ITC) proved tonbe more effective than triphenyltin isothiocyanate (TPT-ITC). Furthermore, the effect of natural (9cRA) and synthetic RXR ligands, both chloride and isothiocyanate derivatives, on vimentin expression was compared.

Název v anglickém jazyce

Down-regulation of vimentin by triorganotin isothiocyanates—nuclear retinoid X receptor agonists: A proteomic approach

Popis výsledku anglicky

An attempt has been made to delineate the role of natural and synthetic retinoid receptor ligands on vimentin expression in the human triple-negative breast cancer cells. The effects of currently synthesized triorganotinnderivatives of the general formula R3SnX (R is butyl or phenyl, X is isothiocyanate), which are considered RXR igands, were investigated in the human MDA-MB-231 breast cancer cell line. Studies were evaluated in thenpresence and absence of all-trans retinoic acid (ATRA), a natural RAR ligand. Vimentin represents the major protein associated with epithelial-mesenchymal transition (EMT), an essential process when the primary tumourntransforms into a malignant one. mRNA and proteomic data obtained in this study, based on the PDQuest software protein evaluation and further quantification of proteins by iTRAQ analysis, suggest that vimentin wasnsignificantly reduced in the combination of RAR ligand and RXR ligand treatment. Both tested triorganotin compounds showed similarly reduced expression of vimentin, but tributyltin isothiocyanate (TBT-ITC) proved tonbe more effective than triphenyltin isothiocyanate (TPT-ITC). Furthermore, the effect of natural (9cRA) and synthetic RXR ligands, both chloride and isothiocyanate derivatives, on vimentin expression was compared.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Toxicology Letters

ISSN

0378-4274

e-ISSN

—

Svazek periodika

318

Číslo periodika v rámci svazku

JAN

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

8

Strana od-do

22-29

Kód UT WoS článku

000496792900003

EID výsledku v databázi Scopus

2-s2.0-85074143518