Comparison of oligosaccharide labeling employing reductive amination and hydrazone formation chemistries

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F20%3A00522452" target="_blank" >RIV/68081715:_____/20:00522452 - isvavai.cz</a>

Výsledek na webu

<a href="http://hdl.handle.net/11104/0306941" target="_blank" >http://hdl.handle.net/11104/0306941</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201900475" target="_blank" >10.1002/elps.201900475</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of oligosaccharide labeling employing reductive amination and hydrazone formation chemistries

Popis výsledku v původním jazyce

In this work, we compare labeling by two negatively charged fluorescent labels, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) and 8-(2-hydrazino-2-oxoethoxy)pyrene-1,3,6-ntrisulfonic acid (Cascade Blue hydrazide [CBH]). Effectiveness of the labeling chemistries were investigated by 4-hydroxybenzaldehyde and maltoheptaose followed by LC/UV-MSnand CE/LIF analysis, respectively. The reaction yield of APTS labeling was determined to be only ∼10%. This is due to reduction of almost 90% of the analyte by sodium cyanoborohydridento alcohol, which cannot be further labeled via reductive amination. However, the CBH labeling provides ∼90% reaction yield based on the LC/UV-MS measurements. Thensignificantly higher labeling yield was also confirmed by CE/LIF measurements. Finally, the more effective hydrazone formation technique of CBH was characterized and appliednfor N-linked glycan analysis by CE/LIF.

Název v anglickém jazyce

Comparison of oligosaccharide labeling employing reductive amination and hydrazone formation chemistries

Popis výsledku anglicky

In this work, we compare labeling by two negatively charged fluorescent labels, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) and 8-(2-hydrazino-2-oxoethoxy)pyrene-1,3,6-ntrisulfonic acid (Cascade Blue hydrazide [CBH]). Effectiveness of the labeling chemistries were investigated by 4-hydroxybenzaldehyde and maltoheptaose followed by LC/UV-MSnand CE/LIF analysis, respectively. The reaction yield of APTS labeling was determined to be only ∼10%. This is due to reduction of almost 90% of the analyte by sodium cyanoborohydridento alcohol, which cannot be further labeled via reductive amination. However, the CBH labeling provides ∼90% reaction yield based on the LC/UV-MS measurements. Thensignificantly higher labeling yield was also confirmed by CE/LIF measurements. Finally, the more effective hydrazone formation technique of CBH was characterized and appliednfor N-linked glycan analysis by CE/LIF.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA18-00062S" target="_blank" >GA18-00062S: Vysoce citlivá analýza glykoproteinů pomocí nových značek pro fluorescenční detekci a hmotnostní spektrometrii s elektrosprejovou ionizací</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

—

Svazek periodika

41

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

684-690

Kód UT WoS článku

000513664800001

EID výsledku v databázi Scopus

2-s2.0-85079716358